摘要
目的研究慢性病毒性肝炎患者血浆血管活性肠肽水平（VIP）变化，以及与血清细胞因子的关系，探讨在VIP慢性肝炎发病机制中的作用。方法选择病例共分为两个实验组：组Ⅰ为正常对照组，组Ⅱ为慢性肝炎组（包括18例慢性乙型肝炎患者和20例慢性丙型肝炎患者）。ELISA方法检测血浆中VIP水平，检测血清中IL-6、IL-18、γ干扰素（IFN-γ）水平以及丙型和乙型肝炎病毒标志物；生化分析仪检测肝功能指标；荧光定量聚合酶链和逆转录荧光定量聚合酶链反应检测血浆中HBV DNA和HCV RNA。结果组Ⅰ和组Ⅱ血浆VIP的表达分别为（33±4）pg／mL、（21±5）pg/ml，慢性肝炎患者血浆VIP水平明显降低，差异具有统计学意义（P〈0.01）；血清IL-6、IL-18、IFN-γ水平高于对照组，差异具有统计学意义（P〈0．01）。结论VIP在慢性病毒性肝炎的病毒清除和肝脏损伤中发挥重要作用，可能通过调节费．痘系统释放细胞因子发挥作用．VIP可以反映体内的免疫状态．
Objective To investigate the possible alterations of vasoactive intestinal peptide （VIP） in plasma with chronic hepatitis patients. Methods Individuals that took part in the experiment were divided into two groups ： group Ⅰ （ normal control） , group Ⅱ （ including 18 chronic hepatitis B patients and 20 chronic hepatitis C patients）. The makers for HBV and HCV, virological evaluation （HBV DNA and HCV RNA） and serum alanine aminotransferase （ALT） were performed. VIP in plasma was detected by ELISA methods. IL- 6, IL- 18 and IFN -γin serum were detected by ELISA methods. Results The levels of VIP in plasma were significantly lower （ P 〈0.01 ） and the levels of IL - 6 ,IL - 18 and IFN -γin serum were significantly higher （ P 〈 0.01 ） with chronic hepatitis patients than with normal control. Conclusion The changes of VIP in plasma from chronic hepatitis B patients suggest VIP take part in alteration of T - cellular immune response and liver injury. VIP modulates immunological function by regulate inflammatory cytokines.
出处
《中原医刊》
2007年第14期16-17,共2页
Central Plains Medical Journal
关键词
慢性肝炎
血管活性肠肽
免疫
Chronic hepatitis
Vasoactive intestinal peptide
Immune
