摘要
使用反相高效液相色谱和二极阵列检测及大气压化学电离质谱技术对玉米蛋白粉中叶黄素、玉米黄质和隐黄质及其主要顺式异构体进行分离和鉴定。以体积分数95%的乙醇和四氢呋喃按体积比1∶1混合为提取溶剂,采用Carotenoid C30色谱柱（250.0 mm×4.6 mm,5μm）分离,甲醇和丙酮为流动相,流速0.5 mL/min,检测波长450nm,采用DAD和APcI-MS技术对叶黄素、玉米黄质和隐黄质的主要顺式异构体进行鉴定。结果表明：叶黄素、玉米黄质和隐黄质及这3种色素的异构体在60 min内较好分离,其质量浓度为5.0-150.0、2.7-80.1和0.6-18.0μg/mL时与峰面积线性关系良好,相关系数分别为0.999 7、0.999 3和0.999 5;该检测方法精密度的相对标准偏差（RSD）为0.11%-0.39%,回收率为95.95%-101.78%。HPLC检测方法适用于玉米蛋白粉等干植物原料中叶黄素类色素的精确定量。
A gradient reversed-phase high performance liquid chromatography （RP-HPLC） coupled with UV-vis photodiode array detection （DAD） and APcli-MS was developed for separation of lutein, zeaxanthin, β-cryptoxanthin, and their isomers from pigment extracts of corn gluten meal. The pigments were extracted using the solvent mixture of 9.5% ethanol （by volume） and THF by volume ratio of 1/1. Chromatographic separation was performed in Carotenoid C30 column （250 mm× 4.6 mm ID., 5 μm） at ambient temperature with an eluent consisting of methanol and acetone at a flow rate of 0.5 mL/min, the eluate was monitored at 450 nm. The result showed that separation and identification of lutein, zeaxanthin, β-cryptoxanthin and their isomers were completed in 60 min. The linear ranges of lutein, zeaxanthin and β-cryptoxanthin were 5-150 μg/mL （R^2 = 0. 999 7）, 2.7-80.1 μg/mL （R^2 = 0.999 3） and 0.6- 18.0 μg/mL （R^2 = 0. 999 5）, respectively. This method is precise and accurate （RSD 0.11% - 0.39 % ）, the average recoveries ranged from 95.95 % to 101.78 % and can be used for the precise quantification of lutein and other pigments of dry plant material such as corn gluten meal.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2007年第4期61-66,共6页
Journal of China Agricultural University
基金
北京市科技计划项目课题(Y0704003040511)
