摘要
目的本研究通过加铁和去铁干预试验,了解不同铁状态下IRP2在人白血病HL-60细胞中的表达情况。方法将FeCl3或去铁胺(Desferrioxamine,DFO)加入含有体积分数10%胎牛血清的RPMI-1640培养液中,使HL-60细胞处于缺铁或富铁的不同状态下培养。于细胞培养后第12、24和48 h收集各组细胞,提取细胞总RNA。采用RT-PCR半定量法测定IRP2mRNA的相对表达量。结果各实验组之间IRP2mRNA的表达量变化不大,组间差异无统计学意义(F=1.199,P>0.05),但随细胞培养时间的延长,IRP2mRNA的表达降低,组内差异有统计学意义(F=43.418,P<0.05)。结论细胞内铁水平状态可能不影响IRP2mRNA的表达。
Objective To investigate the expression levels of IRP2mRNA under different iron circumstances of HL-60 cells. Methods HL- 60 cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum which was treated with ferric chloride（ FeCl3 ） or deferoxamine（DFO）. The cells were harvested at 12,24 and 48h proliferation, and total RNA was isolated; cDNA was synthesized by reverse transcription （ RT）, and relative amounts of IRP2 mRNA were determined by RT-PCR. Results The levels of IRP2 mRNA remained constant in all cells. There wasn＇ t significant difference among the subjects（ F = 1. 199, P 〉 0.05 ）. However, the expressions of IRP2 mRNA were decreased when the time of cell culture was prolonged. There was significant difference among the subjects （F = 43. 418, P 〈 0.01 ）. Conclusions The levels of iron in cells might not affect the expressions of IRP2 mRNA in HL-60 cells.
出处
《肿瘤基础与临床》
2007年第4期299-300,共2页
journal of basic and clinical oncology
