摘要
目的:观察骨髓基质干细胞与异种脱蛋白松质骨复合修复尺骨缺损的可行性。方法:实验于2006-01在辽宁医学院附属第一医院外科实验室(省级重点实验室)完成。①实验材料:取12月龄健康杂种犬18只,体质量(15.0±2.2)kg。②实验方法:将所有动物的双侧尺骨制备成中断20mm骨-骨膜缺损模型,骨髓基质干细胞与脱蛋白松质骨于体外复合培养后,将其植入其中16只犬的右侧缺损处作为实验组,左侧植入单纯脱蛋白松质骨作为对照组,另2只犬不植入任何材料为空白组。③实验评估:在4,8,12周分别行大体标本、扫描电镜和组织学观察,比较3组骨缺损修复的能力。结果:纳入健康杂种犬18只,均进入结果分析。4周时实验组支架材料部分吸收,植入物表面有纤维骨痂形成,对照组支架材料少量吸收,植入物表面有少量骨样组织形成;8周时,大体标本及组织学观察,实验组中的支架材料已完全降解,骨缺损部分修复,对照组中植入物两端少量新骨形成,材料中为纤维骨样组织;12周时,实验组骨缺损完全修复,对照组植入物两端有新骨包绕,与骨端连接紧密。12周时空白组骨缺损未修复。结论:利用体外扩增培养的骨髓基质干细胞与异种骨组合,具有较强的骨传导和骨诱导活性。
AIM： To investigate the feasibility of repairing ulna defect with bone marrow stromal cells （BMSCs） and xenogeneic deproteinised cancellous bone （XDCB）.
METHODS： The experiment was performed at the Laboratory of General Surgery, First Affiliated Hospital, Liaoning Medical University in January 2006. ①Eighteen healthy hybrid dogs aged 12 months and with the body mass of （15.0±2.2） kg were selected. ②The 20 mm bone-periosteum defect models were made in bilateral ulna of all dogs. MSCs were co-cultured with XDCB in vitro, and implanted in fight defect region of 16 dogs as experimental group, whereas only XDCB was implanted in left side of dogs as control group. The other dogs did not receive any intervention as blank group. ③General sample observation, scanning electron microscope and histological observation were performed at weeks 4, 8 and 12, respectively and the repair capability was assessed in the 3 groups.
RESULTS： A total of 18 healthy hybrid dogs were involved in the result analysis. The XDCB was partially absorbed and fibrous callus formed in the surface of graft in the experimental group, and the XDCB was rarely absorbed and rare osteoid tissues appeared in the control group at week 4. General sample and histological observations showed that the XDCB was fully degraded and partial bone defect was repaired in the experimental group, whereas few new bones formed and fibrous bone tissue appeared in the control group at week 8. Bone defect was repaired in the experimental group, whereas new bones around graft were seen and were closely connected with extremities in the control group at week 12. The defects were not repaired at week 12 in the blank group.
CONCLUSION： Combination of BMSCs and XDCB has strong bone conduction and bone induction.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第32期6330-6333,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
辽宁省教育厅攀登计划资助项目(20071019)~~
