摘要
利用扫描电镜对12个分布于我国的百合Lilium种(包括2个变种)及6个栽培品种进行花粉形态观察与比较研究。结果表明,百合属花粉粒为椭圆体至长椭圆体,萌发沟均为单萌发沟,萌发沟长达两端。表面纹饰网纹,网眼为不规则多边形至近圆形,大小不一。网脊由瘤状或盘珠状颗粒较紧密排列而成,单排基柱,脊宽1.17-2.48μm,有时有断点。网眼内有疣状、瘤状或棒状突起,少数品种无突起。在花粉形态性状比较基础上,初步探讨了百合属植物的系统进化关系。通过对供试18个种、变种及品种的5个花粉形态指标进行聚类分析,结果除卷丹L. lancifolium外,较准确地将形态分类上的百合组与卷瓣组聚为2个类群。
China is the natural distributional center of Lilium,however,more than half of the wild species still remains to be exploited.Researches on wild resources is an important base to breed new cultivars.Pollen morphology of 12 wild Lilium species(including 2 varieties,distributed in the south of China)and 6 cultivars(5 cultivars of Asiatic hybrids,1 cultivar of Longiflorum hybrids)had been observed and compared by means of SEM(scanning electron microscope).The results show that the pollen grains of lily are generally ellipsoid or long ellipsoid with mono-culpus and reticulate sculptures.Culpus reaches to the two poles.Apertures of reticulate sculptures is erose or approximately rotundity with different width.The granules look like nubble or wart and are closely arranged to reticulate spine.The width of spine is 1.17-2.48 μm,and is breaken sometimes.There are protuberances in apertures of reticulate,seldom none.The phylogenetic relationships between 12 species and 6 cultivars were discussed based on the pollen morphology.The five pollen morphological index of 12 species and 6 cultivars were clustered.The results show that the species and cultivars had been sorted to two kinds,section Lilium and section Sinomartagon except of L.lancifolium.
出处
《浙江林学院学报》
CSCD
北大核心
2007年第4期406-412,共7页
Journal of Zhejiang Forestry College
基金
上海市科技兴农重点攻关项目(2004-04-31)
江苏省高校自然科学基金资助项目(05KJB180050)
关键词
植物学
百合
花粉粒形态
种质资源
亲缘关系
扫描电镜
聚类分析
botany
Lilium
pollen morphology
germplasm resource
genetic relationship
scanning electron microscope(SEM)
cluster analysis