摘要
应用地高辛标记的人β神经生长因子ＤＮＡ探针原位杂交组化技术，研究雌性ＩＣＲ小鼠颌下腺的神经生长因子（ＮＧＦ）基因表达。颌下腺经４％多聚甲醛固定，常规冰冻切片，并以同种雄性小鼠颌下腺作阳性对照。实验结果显示，雌性小鼠颌下腺的ＮＧＦｍＲＮＡ分布与雄性小鼠相同，主要存在于纹状管和颗粒曲管上皮细胞中，但杂交信号明显弱于雄性小鼠；在原位杂交反应阳性的纹状管或颗粒曲管上皮细胞之间常可见到几个阴性细胞，阳性管道的密度亦显著低于雄性小鼠。研究表明，小鼠颌下腺ＮＧＦ基因的表达受到雄激素的调节，这种调节作用可能发生于转录水平。ＮＧＦ基因转录少，纹状管或颗粒曲管部分上皮细胞ＮＧＦ基因不开放以及颗粒曲管不发达是雌性小鼠颌下腺ＮＧＦ含量明显低于雄性小鼠的主要原因。
The nerve growth factor(NGF) gene expression in submandibular gland of female mice was studied by in situ hybridization with digoxigenin-lebelled human β NGF DNA probes. The adult ICR mice were perfused with 4% paraformaldehyde in phosphate buffer. The submandibular glands were removed and immersed in the same fixative. The specimens were sectioned on a cryostat. The results showed that in submandibular gland of female mice NGF mRNA was mainly localized in the secretory striated ducts and granular convoluted tubules as same as in male mice. But the hybridized signal was weaker and the density of positive tubules was lower . Several negative cells were usually found among the positive epithelial cells of secretory striated ducts and granular convoluted tubules. These results demonstrated that NGF gene expression in the submandibular glands of mice was regulated by androgen in transcription level. Low expressed NGF mRNA and poor-developed granular convoluted tubules show that NGF concertrations in submandibular gland of female mice are lower.
出处
《南通医学院学报》
1997年第2期147-149,共3页
ACTA Academiae Medicinae Nantong
基金
国家杰出青年科学基金
关键词
神经生长因子
颌下腺
原位杂交
雌性
MRNA
nerve growth factor(NGF) submandibular gland in situ hybridization female mouse
