摘要
目的探讨二氧化硅(SiO2)是否通过影响肺泡巨噬细胞(AM)和肺成纤维细胞(FB)中转化生长因子β(TGF-β1)的表达而参与矽肺纤维化的发生发展。方法以支气管肺泡灌洗法收集大鼠AM,在体外用含有SiO2(50μg/ml)的DMEM培养基和不含SiO2的DMEM培养基培养2,6,12,18,24,36 h,然后收集培养18 h的AM上清液。用胰酶消化法分离培养大鼠FB,与AM上清液共同孵育6,12,18,24,36和48 h,用免疫细胞化学的方法检测AM及FB中TGF-β1的表达。结果经SiO2刺激的大鼠AM中TGF-β1的表达与空白对照组比较增加,在6,12,18 h 3个时间点差异有统计学意义(P<0.05,平均吸光度A值分别为0.118±0.008 vs 0.107±0.008,6 h;0.135±0.010 vs 0.119±0.015,12 h;0.143±0.012 vs0.125±0.015,18 h);AM上清液也可使FB中TGF-β1的表达上调,经体外再次染尘的AM上清液作用更为明显,与空白对照组比较,在各个时间点差异均有统计学意义(P<0.01)。结论在本试验条件下,经体外细胞培养,显示SiO2可通过上调AM和FB中TGF-β1的表达,增加胶原合成而参与矽肺纤维化的发展。
Objective To study the effect of SiO2 on the expression of transforming growth factor-β1 （TGF-β1） in rat alveolar macrophages （AMs） and lung fibroblasts （FBs）. Methods Rat AMs were collected by bronchoalveolar lavage and incubated in vitro in the DMEM medium containing SiO2 （50μg/ml） and DMEM medium without SiO2 for different time periods （2, 6, 12, 18, 24, and 36 h）. Then the AM supernatant incubated for 18 h was collected. The rat fetal lung FBs were isolated by trypsin dispersion method, and incubated with AM supernatants for 6, 12, 18, 24, 36, and 48 h. Immunocytochemical method was used to detect the TGF-β1 expression in both rat AMs and lung FBs. Results The TGF-β1 expressions in rat AMs exposed to silica in vitro were regulated upward, the values at 6, 12, and 18 h were statistically different （P〈0.05, mean absorption A values were 0. 118 ±0. 008 vs 0. 107 ±0. 008 for 6 h： 0.135±0.010vs0.119±0.015 for 12 h; and0.143±0.012 vs0.125±0.015 for 18 h）. AMsupernatant could also make the TGF-β1 expressions in FBs going upward. It was more obvious in AM supernatants reexposed to silica in vitro. When comparing with the controls, the values from all time periods were all statistically significant （P〈0.01）. Conclusions Experiment results showed that SiO2 could induce the increase of collagen synthesis so as to participate the silicotic fibrosis through regulating the TGF-β1 expression in AMs and FBs upward.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2007年第4期214-217,共4页
Industrial Health and Occupational Diseases
