摘要
With the completion of the Populus genome sequence in 2006,the study on functional genomics in Populus has become a major task.Establishment of Populus mutant population is an essential approach for forestry functional genomics study.The activation tagging is a promising and powerful method to construct mutant library of Populus and to further discover new gene and elucidate its function now.A novel T-DNA activation tagging pCAS05 was constructed for forest functional genomics in this study.We could select conveniently and easily transgenic plants by spraying Basta because bar gene is constructed in this vector as a selection marker.Moreover,we could also construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bar gene.
With the completion of the Populus genome sequence in 2006, the study on functional genomics in Populus has become a major task. Establishment of Populus mutant population is an essential approach for forestry functional genomics study. The activation tagging is a promising and powerful method to construct mutant library of Pop- ulus and to further discover new gene and elucidate its function now. A novel T-DNA activation tagging pCAS05 was constructed for forest functional genomics in this study. We could select conveniently and easily transgenic plants by spraying Basta because bar gene is constructed in this vector as a selection marker. Moreover, we could also construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bar gene.
出处
《林业科学研究》
CSCD
北大核心
2007年第4期586-590,共5页
Forest Research
基金
国家林业局"948"项目"安全选择标记基因及林木转基因新技术引进"(2005-4-58)部分内容
