摘要
从海水中筛选产DHA的微生物,共采集海水样品280余份,用苏丹黑染色法得到160株产油脂菌株,在对60株脂肪粒较大的微生物用索氏抽提法提取油脂后,初筛得到油脂含量高于8%的菌株7株。对10株油脂产量较高的菌株进行复筛,编号7-3的菌株油脂含量达到15.9%,DHA在油脂中的含量达到45.2%,选用7-3作为目的菌株。对7-3进行形态特征、培养特征及生理生化特征鉴定,初步判定菌株7-3为酒香酵母属(Brettanomyces sp.)。
Microbes that produce Docosabexaenoic Acid were isolated from seawater. 160 strains capable of producing lipids were screened out using Sudan Black B dying method from 280 seawater samples. From 60 strains of microorganisms producing bigger lipid particles, 7 strains of them capable of producing lipids more than 8% were obtained with Soxhlet abstracting method in the first screening. In the secondary screening from 10 strains with high lipids yield, strain 7-3 capable of producing 15.9% lipids was obtained, in which the content of DHA（ Docosabexaenoic Acid）is 45.2%. Strain 7-3 was identified as Brettanomyces based on its morphological properties, cultural characteristics, physiological and biochemical properties.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第4期749-752,共4页
Microbiology China
基金
江苏省教育厅项目(No.04KJD240024)
关键词
酒香酵母
DHA
分离筛选
鉴定
Torulopsis, Docosahexaenoic Acid, Screening, Identification
