摘要
建立了高效液相色谱法（HPLC）测定保健食品中姜黄素含量的方法。用甲醇对样品进行超声提取，以ZORBAX XDB-C18（5μm，4．6×250mm）为分析柱，乙腈：磷酸盐缓冲液=50：50为流动相，流量1.0mL·min^-1,测波长为425nm，姜黄素在8～500ng范围内呈良好线性，相关系数为0．99999，检出限为0.1ng；回收率在97.8％～102．1％之间。本方法操作简便、快速，结果准确、可靠，可作为保健食品中姜黄素的质量控制监测。
The method for the determination of curcumin in health food by HPLC was established. The sample was extracted in methyl alcohol through supersonic treatment. The analysis column was ZORBAX XDB-C18（5 μm, 4.6 × 250 mm）. The mobile phase was （ 1 ： 1 ） acetonitrile-phosphate buffer solution. The flow rate was 1.0 mL·min^-1. The UV detector was set at 425nm. A good linear relationship was obtained between the concentration of 8- 500 ng, r = 0. 99999, the detect limit was 0.1 ng. The recovery of curcumin was in the range of 97.8 % - 102.1%. This method was simple, accurate and reliable, and could be used for the quality control of curcumin in health food.
出处
《化工技术与开发》
CAS
2007年第8期38-39,共2页
Technology & Development of Chemical Industry
