摘要
目的测定眼镜蛇毒细胞毒素（ＣＶ－ＣＴＸ）对人癌细胞株的细胞抑制作用，以期能简便准确地反映其抗癌活性。方法应用溴化二苯四偶氮盐（ＭＴＴ）法测定在ＣＶ－ＣＴＸ作用下体外培养的人胃癌细胞株（ＳＧＣ－７９０１）和人肝癌细胞株（ＳＭＣ）的存活情况和其量效关系。结果（１）ＳＭＣ、ＳＧＣ－７９０１的活细胞数与其分解ＭＴＴ的量成正线性关系。（２）ＣＶ－ＣＴＸ对ＳＭＣ、ＳＧＣ－７９０１癌细胞株的细胞抑制作用呈良好的量效关系，ＩＣ５０分别为２．４５和１．２４μｇ／ｍｌ。结论（１）用ＭＴＴ法测定癌细胞的增殖和衰减是可靠的。（２）应用ＭＴＴ法证实ＣＶ－ＣＴＸ对体外培养的人癌细胞的杀伤程度可作为其抗癌活性指标。
Objective\ CV CTX is a cytotoxin separated from CV (cobra venom,CV).\ To reflect precisely the anticancer activity of CV CTX on human cancer cell lines.\ Methods\ To study the effect of CV CTX on the survival rate of cultured human cancer cells and dose effect relationship by 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium bromide(MTT) assay method.\ \ Results\ (1)The amount of living cultured cancer cells was well correlated with the amount of decomposed MTT.(2)The inhibition of CV CTX on SMC and SGC 7901 cell lines showed good dose effect relationship,the IC 50 was 2.45 and 1.24 μg/ml respectively.\ Conclusion \ (1)The MTT assay is a trust worthy method for testing the proliferation and attenuation of cancer cells.(2)The killing rate of CV CTX on the cultured cancer cell lines by using MTT assay was validated as an index of anticancer activity.
出处
《福建医科大学学报》
1997年第1期17-19,共3页
Journal of Fujian Medical University
关键词
眼镜蛇毒
细胞毒素
胃癌
癌细胞株
肝癌
cobra venom cytotoxin
3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium bromide
human stomach cancer cell line
human liver cancer cell line
