摘要
从浙江临安、桐庐发生青枯病的桑树的根、茎部分经分离纯化得217个桑树青枯病菌分离物,从217个分离物菌株中选择16个典型菌株进行研究,经离体水培接种试验,表明这些分离物均引起桑枝"凋萎"症状,其形态、大小、染色反应、培养性状与对照番茄青枯菌相近,但生长速度较慢。参照Haywind的标准,这些菌株均为雷尔氏菌的生理小种1,75%为青枯菌的生化型Ⅴ,25%为青枯菌的生化型Ⅲ。
Two hundred and seventeen bacterial strains of Ralstonia solanacearum were isolated from the xylem and phloem of roots and stems infested by mulberry bacterial wilt in Lingan and Tonglu counties in Zhejiang province. Sixteen typical strains from 217 bacterial strains were studied.The pathogenicity test found that they could cause the wilt of mulberry and were the similar to that of R. solanacearum of tomoto in shape, size, dye-reaction etc. Acceding to Haywind＇ s classification criterion of R. solanacearum, the strains were classified as the physiological race 1. Biochemistry test of the strains showed that 75 % of R. solanacearum was biovar Ⅴ ,25 % of R. solanacearum was biovar Ⅲ.
出处
《浙江农业学报》
CSCD
2007年第4期306-309,共4页
Acta Agriculturae Zhejiangensis
基金
浙江省重大科研攻关项目(2006C12057)
关键词
桑树
青枯病
生理小种
生化型
mulberry
Ralstonia solanacearum
physiological race
biovar
