摘要
目的建立替米沙坦片的含量测定方法。方法采用反相高效液相色谱法。色谱柱为DiamonsilTM C18柱(5um,4.6mm×250mm);流动相为乙腈-0.5%磷酸溶液(用三乙胺调pH值至6.0)(52:48);检测波长为297nm;流速为1.0mL.min-1;柱温为30℃;以外标法计算替米沙坦的含量。结果替米沙坦在28.16～140.8μg.mL-1范围内呈良好的线性关系(r=0.9999),平均回收率为100.56%,RSD=0.22%。结论所建立的方法简便、快速、准确,辅料不干扰主药测定,可用于替米沙坦片的含量测定。
Objective To establish a HPLG method to determine the content of telmisartan tablets. Methods RP- HPLC was used in this experiment, Diamonsil^TM C18 （5μm, 4.6mm×250mm） as stationary phase, acetonitrile - 0.5 % phosphoric acid （pH6.0, adjusted by triethylamine） （52：48） as mobile phase with flow rate of 1 mL·min^-1 and detection wave of 297nm. The content of telmisartan tablet was counted by extemal standard method. Results The linear range was 28.16- 140.8μg·mL^-1 （r= 0.9999）, The average recovery was 100.56%, and RSD=0.22%. Conclusion The method is simple, rapid and accurate, and is not interfere by excipients. It can be used for the quality control of telmisartan tablets.
出处
《实用预防医学》
CAS
2007年第4期1223-1225,共3页
Practical Preventive Medicine
