摘要
合成了O-（硫杂蒽酮-[2]-基）-氧乙酸,并用元素分析、IR、^1H NMR和13C NMR等方法对其结构进行了表征。用光谱学方法研究了该化合物与ct-DNA的相互作用。结果表明,在pH 7.38的模拟体液条件下,该化合物的紫外-可见吸收光谱随ct-DNA浓度的增加,表现出减色效应,使ct-DNA的圆二色谱正负峰的吸收强度有所降低。该化合物的荧光强度被ct-DNA显著猝灭,猝灭常数为1.25×10^4L·mol^-1,当ct-DNA的浓度在0-12.0 mg·L^-1范围内变化时,荧光猝灭值与ct-DNA的浓度呈现良好的线性关系。该化合物对ct-DNA的检出限为1.63 mg·L^1。据此,O-（硫杂蒽酮-[2]-基）-氧乙酸是一种可用于测定ct-DNA浓度的新试剂，与ct-DNA的作用有嵌入和静电吸引两种方式。
The thiaxanthon-2-yl-oxyacetate was synthesized, and its structure was characterized by elemental analysis, IR,^1 H NMR, and ^13C NMR spectra. It was found from the experiment that thiaxanthon-2-yl-oxyacetate had very intense fluorescence, so spectroscopy methods were used to study the interactions of thiaxanthon-2-yl-oxyacetate and ct-DNA. The ultraviolet absorp- tion intensity of thiaxanthon-2-yl-oxyacetate decreased as the concentration of ct-DNA increased at pH 7.38. There was some de- based intensity both in the negative and positive spectra of CD spectra of ct-DNA when thiaxanthon-2-yl-oxyacetate interacted with ct-DNA, and quenching effect occurred in the fluorescence spectrum of thiaxanthon-2-yl-oxyacetate. The quenching constant is 1.25×10^4L·mol^-1, when the concentration of ct-DNA changed from 0 to 12.0 mg ·L^-1, and the connection between the quenching data and the concentration of ct-DNA shows a favorable linear relationship. The detection limit was 1.63 mg·L^-1 , so the compound is a new reagent for the measurement of ct-DNA. The interaction of TYO and ct-DNA was insetted and static.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第8期1580-1583,共4页
Spectroscopy and Spectral Analysis
基金
甘肃省自然科学基金项目(3ZS051-A25-021)
甘肃省高分子材料重点实验室项目资助
关键词
合成
表征
光谱学
CT-DNA
-基)-氧乙酸
Synthesis
Characterization
Spectroscopy
ct-DNA
Thiaxanthon-2-yl-oxyacetate
