期刊文献+

STI571诱导Jurkat细胞表达Th1细胞因子及HLA-DR、CD-25

下载PDF
导出
摘要 目的应用流式微珠阵列法检测Th1/Th2细胞因子表达及探讨STI571对Jurkat细胞的作用。方法以不同浓度STI571诱导培养Jurkat细胞,流式微珠阵列法检测培养上清液中IL-2、,IL-6、TNF-α,和IFN-γ表达,流式细胞术检测IL-2膜上受体(CD-25)和HLA-DR表达;结果STI571诱导Jurkat细胞IL-2、TNF-α表达且表达量呈浓度依赖形式升高,未检测到IL-6表达,CD-25和HLA-DR表达明显升高。结论STI571能诱导Jurkat细胞高表达Th1细胞因子和CD-25、HLA-DR,流式微珠阵列法能准确监测Th1/Th2细胞因子表达的动态变化。
出处 《浙江实用医学》 2007年第4期247-249,共3页 Zhejiang Practical Medicine
  • 相关文献

参考文献5

  • 1Hodge G,Hodge S,Haslam R,et al.Rapid simultaneous measurement of multiple cytokines using 100 microl sample volumes-association with neonatal sepsis.Clinical & Experimental Immunology,2004,137:402
  • 2Khan SS,Smith MS,Reda D,et al.Multiplex bead array assays for detection of soluble cytokines:comparisons of sensitivity and quantitaive values among kits from multiple manufacturers.Cytometry B Clin Cytom,2004,61:35
  • 3Aoe K,Hiraki A,Murakami T,et al.Relative abundance and patterns of correlation among six cytokines in pleural fluid measured by cytonetric bead array.Int J Mol Med,2003 Aug,12:193-8
  • 4Morgan E,Varro R,Sepulveda H,et al Cytometric bead araray:a multiplexed assay platform with applications in various areas of biologoy.Clin lmmunol,2004,110:252
  • 5Attila Tarnok,Jorg Hambsch,Roy Chen,et al.Cytometric Bead Array to Measure Six Cytokines in Twenty-Five Microliters of Serum.Clinical Chemistry,2003,49:1000

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部