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Apelin-13对离体缺血/再灌注大鼠心脏损伤的保护性影响 被引量：1

Protective effects of apelin-13 on ischemia/reperfusion injury of rat heart
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摘要 目的观察apelin-13对离体大鼠缺血/再灌注心脏损伤的影响,并初步探讨其对apelin受体APJ(putative re-ceptor protein related to the angiotensin receptorAT1)及细胞信号Akt1/2的影响。方法Langendorff装置恒流灌注大鼠离体心脏,采用停灌/复灌方式复制缺血/再灌注模型;观察缺血/再灌注期间心脏收缩期左心室内压上升的最大变化速率(+LVdp/dtmax)及舒张期左心室内压下降的最大变化速率(-LVdp/dtmax);RT-PCR和Western blot测定组织中APJ受体mRNA和Akt1/2蛋白的表达情况。结果Apelin-13可以增加缺血/再灌注损伤心脏的±dp/dtmax(P<0·01);可以明显增强心肌组织中Akt1/2的表达;缺血/再灌注可以引起心肌组织中APJ受体表达上调。结论Apelin-13拮抗缺血/再灌注引起的心脏收缩及舒张功能障碍;可能与组织中APJ受体表达上调引起Akt1/2表达增强有关。 Objective To observe the protective effects of apelin-13 on on the expression of APJ （putative receptor protein related to the angiotensin receptor AT1 ） and Akt1/2 in ischemia/reperfusion injury rat heart. Methods Langendorff device was used to perfuse isolated rat heart with constant flow. The ischemia/reperfusion models of rat heart were made by stopping and reperfusion KH fluid to the heart; observing the change of maximal rate of the pressure increase（ ＋ dp/dmax） and maximal rate of the pressure decrease（-dp/dmax） of left ventricle in rat heart. RT-PCR was used to assay the expression of APJ mRNA in rat heart, Western blot was used to assay Akt1/2 protein in rat heart. Results The groups given apelin-13 had higher ＋ LVdp/dmax（P 〈0. 01 ） ; and resulted in hyperexpression of Akt1/2 protein （ P 〈 0. 05 ）, while the expression of APJ mRNA was up regulated in IR group （P 〈 0. 05）. Conclusion Apelin-13 can resist the functional impairment in rat heart caused by ischemia/reperfusion injury, and the protecting effects of apelin-13 may be explained by in up regulated expression of APJ in rat heart, and the APJ may up regulate the expression of Akt1/2.
作者 曾翔俊 王红霞 芦玲巧 王晓燕 马利权 张立克 唐朝枢
机构地区 首都医科大学病理生理学教研室 北京大学第一医院心血管研究所
出处 《基础医学与临床》 CSCD 北大核心 2007年第8期864-867,共4页 Basic and Clinical Medicine
基金 北京市自然科学基金(7062007) 北京市教育委员会科技发展项目(KM200510025009)
关键词 缺血/再灌注损伤 apelin多肽 心肌 ischemia/reperfusion.injury apelin peptiole myocardium
分类号 R363 [医药卫生—病理学]
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参考文献9

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二级参考文献15

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共引文献9

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基础医学与临床
2007年 第8期

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