摘要
目的研究肺癌患者化疗前后血清自体荧光光谱,探讨其临床意义。方法应用F-4500型荧光分光光度计,激发光波长301nm,测定200～800nm发射光范围内的肺癌患者化疗前后及正常对照组的血清自体荧光光谱。结果①肺癌患者化疗前第3、4、6峰的荧光强度及第3峰与第4峰的荧光强度比值I3/I4高于正常对照组(P<0·01);②肺癌患者化疗后第4、6峰的荧光强度低于化疗前(P<0·01)。结论血清自体荧光光谱测定可望用于肺癌辅助诊断及疗效观察。
Objective To study the autofluorescence spectra of serum in patients with lung cancer during chemotherapy and investigate its clinical value. Methods The autofluorescence spectra of serum in patients with lung cancer and control were scanned with F -4500 spectrophotoflu - orometer,when the exciting wavelength was set to 301nm and the emiting wavelength ranged from 200nm to 800nm. Results （1）the fluorescence intensity of the third ,forth ,sixth peaks and the ratio of the intensity of the third to forth peak I3/I4 in patients with lung cancer before chemotherapy were higher than those of control （ P 〈0. 01 ）;（2）the fluorescence intensity of the forth ,sixth peaks in patients with lung cancer after chemotherapy were lower than those of patients before chemotherapy （ P 〈 0. 01 ）. Conclusion Scan on autofluorescence spectra of serum could be helped for patients with lung cancer in the diagnosis and the observation of the therapeutic effect.
出处
《医药论坛杂志》
2007年第15期1-2,5,共3页
Journal of Medical Forum
基金
河南省科技攻关项目(0524410098)
关键词
肺癌
血清
荧光光谱
Lung neoplasm
Serum
Fluorescence spectra
