摘要
目的检测毛细支气管炎患儿血清白三烯水平及免疫功能指标。方法用酶联免疫吸附试验法检测血清半胱氨酰白三烯水平;散射比浊法检测免疫球蛋白;流式细胞仪用于T细胞亚群检测。结果毛细支气管炎组血清白三烯水平升高,与对照组比较,P<0.05;血清免疫球蛋白A(IgA)水平与对照组比较降低,P<0.05;血清IgM水平升高,P<0.05;血清IgE水平升高,P<0.05;T细胞亚群检测与对照组比较,CD3+CD4+淋巴细胞检测结果差异无统计学意义,CD3+CD8+检测结果升高、CD3+CD4+/CD3+CD8+比值降低,P<0.05。结论部分毛细支气管炎患儿可能存在免疫缺陷,血清白三烯水平升高,血清IgA水平降低,IgE水平升高,有助于呼吸道、肺部感染的形成,可能是引起毛细支气管炎后反复喘息和哮喘的因素之一。
Objective To investigate the clinical significance of serum levels of leukotriene and indices for immune function in children with bronchiolitis. Methods Enzyme linked immunosorbent assay （ELISA） was applied to measuring serum level of leukotriene, nephelometry measuring immunoglobulins, and flow cytometry measuring T lymphocyte subpopulation. Results As compared with those of control group, the serum concentraions of leukotriene, IgA, IgM, IgE, and the percentage of CD3＋CD8＋ cells were significantly higher （P〈0.05） in bronchiolitis group. The ratio of CD3＋CD4＋ to CD3＋CDS＋ ratio was higher markedly in bronchiolitis group than that in control group （P〈0.05）. There was no significant difference of CD3＋CD4＋ percentage between bronchiolitis group and control group. Conclusion Partial children with bronchiolitis were immune deficient. Elevated serum level of leukotriene and IgE and low level of serum IgA contribute to development of respiratory and pulmonary infection, as well as repeated pursiness and asthma complicated by bronchiolitis.
出处
《检验医学与临床》
CAS
2007年第9期801-802,共2页
Laboratory Medicine and Clinic
