摘要
目的探讨β-七叶皂甙钠(SA)对脊髓损伤大鼠的功能保护作用。方法120只SD大鼠随机分为四组(n=30)。空白对照组施行椎板切除但不损伤脊髓;其余三组均采用改良Allen法制备大鼠脊髓损伤模型(10 g×10 cm),并于术后分别腹腔注射等体积的生理盐水(对照组)、SA(5.0 mg/kg)(SA组)和甲基强的松龙(100 mg/kg)(MP组),每天给药1次。分别于术后8、24、96 h及7、14 d,运用Rivlin斜板功能障碍率评价动物后肢运动功能变化。处死大鼠取损伤节段标本行组织病理学检查。结果随着观察时间的延长,各组大鼠的脊髓功能均有不同程度的恢复。对照组大鼠术后96 h时出现功能恢复,但随后无进一步恢复现象;SA组和MP组大鼠的脊髓功能,于术后96 h时出现明显恢复,且随时间延长获得进一步恢复。SA组和MP组大鼠Rivlin斜板功能障碍率显著低于对照组(P<0.05),而该两组间比较无明显差异(P>0.05)。组织病理学检查显示,空白对照组大鼠未见坏死神经元细胞;SA组和MP组大鼠同一时间点的坏死神经元细胞数目均明显少于对照组(P<0.05)。结论SA对脊髓损伤大鼠的脊髓功能具有保护作用。
To observe the protection effects of sodium β-aescinate （SA） on the nervous function in the rats with early spinal cord injury （SCI）. Methods One hundred and twenty SD rats were randomly divided into four groups（n = 30）. Rats in the blank control group were performed laminectomy only, while those in the other three groups were injured at the level of T11 spinal segment by Alien＇ s weight drop method （10 g x 10 cm） and immediately intraperitoneally given normal saline（5.0 mg/kg） （control group） , SA（5.0 mg/ kg） （SA group） and methylprednisolone（100 mg/kg） （MP group） once daily, respectively. After 8 h, 24 h, 96 h, 7 d and 14 d, spinal cord function change of posterior limb were determined with Rivlin method. The rats were sacrificed and the injured segments were resected for pathological analysis. Results As time prolonged, the rehabilitation of spinal cord function with various degree could be observed in each group. Function rehabilitation was found among the rats in the control group, SA group and MP group 96 h after injury, and more rehabilitation was gained later in the latter two groups, while that was not the case in the control group. Rats in the SA and MP group gained more significant rehabilitation than those in the control group （P 〈 0.05） , while no significant difference was observed between the two groups（P 〉0.05）. It was revealed by pathological analysis that no necrotic neurons was found in the blank control group, and the necrotic neurons in the SA group and MP group were significantly less than the control group at the same time points （P 〈 0.05 ）. Conclusion SA has protective effects on the nervous function of rats with spinal cord injury.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2007年第8期913-916,共4页
Journal of Shanghai Jiao tong University:Medical Science
关键词
Β-七叶皂甙钠
脊髓损伤
功能
保护
sodium β-aescinate
spinal cord injury
function
protection
