摘要
运用连续石蜡切片技术和组织学观察方法,对常规孵化出膜后1～33日龄鳜(Siniperca chuatsi)淋巴器官个体发育过程的组织学特点进行研究。结果表明,水温23～25℃时,出膜后第4天,部分仔鱼开始摄食并逐渐转入外源性营养阶段。同样水温条件下,出膜后第3天,出现胸腺原基,然后胸腺迅速淋巴化,能明显分为外区、中区和内区。鳜在出膜时已存在原肾管,原肾管前部分发育为头肾原基,由一些造血干细胞和肾小管组成;后部分发育为中肾和后肾,主要由肾小管组成。随着个体发育,头肾的淋巴细胞数量增多,肾小管数量减少,头肾逐渐由排泄器官转化为淋巴器官。出膜后第5天,观察到脾脏原基,脾脏主要由造血细胞和淋巴细胞组成,淋巴化速度较慢。至出膜后30天左右,体形和淋巴器官的发育情况基本与成鱼相似。鳜淋巴器官原基出现的顺序是头肾、胸腺、脾脏;而这些器官淋巴化的顺序是胸腺、头肾和脾脏。
A histological investigation for development of thymus, kidney, and spleen in mandarinfish, Siniperca chuatsi was made from the first day to the 33rd day after hatching. At 23 - 25 ℃ ,on the fourth day after hatching, some larvae began to feed, and then switched to exterior nutrition period gradually. At the same temperature, thymus was first observed on the third day after hatching, and subsequently became lymphoid organ rapidly. It could not be made out outer zone, middle zone and inner zone clearly until the 25th day after hatching. The pronephric duct was already present just after hatching,and then the anterior part of it developed into primodium of pronephrie kidney, which mainly consisted of haemopoietic stem cells and pronephric tubules. The posterior part of it developed into mesonephros and metanephros, which were mostly composed of pronephric tubules. With the development of mandarinfish, the amount of lymphocytes increased and pronephric tubules reduced, the pronephric kidney changed to immune organ from excretory organ gradually. The spleen appeared firstly on the fifth day after hatching, mainly consisted of haemopoietic cells and lymphocytes, and it became lymphoid organ slowly compared with the thymus and pronephric kidney. About 30 days after hatching, the morphology and histological structure of lymphoid organs were similar to those of the adult fish. The appearance order of lymphoid organ primordia was pronephric kidney, thymus and spleen, while these organs became lymphoid organs in the order of thymus, pronephric kidney and spleen during larval development. [Journal of Fishery Sciences of China, 2007,14（5）：756 -761 ]
出处
《中国水产科学》
CAS
CSCD
北大核心
2007年第5期756-761,共6页
Journal of Fishery Sciences of China
基金
广东省科技计划项目(2001B20501)
国家科技支撑计划课题(2006BAD03B05)
关键词
鳜
淋巴器官
个体发育
组织学
Siniperca chuatsi
lymphoid organs
histology
ontogeny
