摘要
目的:制备葛根素脂质体并研究大鼠对其的口服吸收。方法:利用薄膜分散-超声法制备脂质体,利用超滤法分离游离药物测定包封率,观测脂质体的微观形态与粒径分布;HPLC法测定葛根素脂质体大鼠口服后血浆中的药物含量。结果:脂质体对葛根素的包封率为53.0%,脂质体粒子在电镜下为圆形或椭圆形,粒径范围50-300nm;葛根素脂质体对其水溶液的相对生物利用度为168%。结论:脂质体对葛根素口服吸收有显著促进作用。
Objective： To prepare puerarin liposome and study its oral absorption in rat. Methods： Liposome was prepared through the way of film dispersion-ultrasonic. Free puerarin in liposome suspension was separated from liposome through ultrafiltration, and then encapsulation ratio of liposome was determined. Micro-morphology of liposme particles was observed under electronic transmission microscope. Puerarin concentration in blood was determined by HPLC. Results：The encapsulation ratio of puerarin in liposome was 53 %, and liposome particles were global or elliptical. The diameter range of liposome particies was from 50nm to 300nm. The relative availability of puerarin liposme suspension to puerarin solution was 168%. Conclusion ：Liposome as a drug cartier can enhance the oral absorption of puerarin in rat.
出处
《中药材》
CAS
CSCD
北大核心
2007年第8期970-973,共4页
Journal of Chinese Medicinal Materials
关键词
葛根素
脂质体
包封率
Puerarin
Liposome
Encapsulation ratio
