摘要
目的:观察远志皂苷(Tenuigenin TEN)对过氧化氢(H2O2)所致PC12细胞损伤的保护作用。方法:以H2O2损伤PC12细胞为氧化应激损伤模型,MTT法检测细胞存活率;采用紫外分光光度法测定LDH漏出量,观察细胞的损伤程度;硫代巴比妥酸法测定MDA含量,黄嘌呤氧化酶法测定SOD活性。结果:TEN能明显改善H2O2导致的细胞损伤,与H2O2处理组相比,20、10 mg/L组可使细胞存活率升高,LDH漏出量明显降低,降低MDA含量,提高SOD活性。结论:TEN对H2O2诱导的PC12细胞损伤具有明显的保护作用。
Objective：To study the protective effects of tenuigenin （TEN） on the PC12 cells injury induced by H2O2. Methods： Oxidative damage PC12 cells model was induced by H2O2. The survival rate of the cells was determined by MTT method. The content of LDH was determined by ultraviolet spectrophometry and the content of MDA and SOD activity was measured respectively by thiobarbitu-tic aicd and xanthine oxidese method Results ：tenuigenin could reduce obviously cells injury induced by H2O2. 10 mg · Ll tenuigenin could improve the cells survival rate, reduce LDH releasing and MDA content, increase SOD activity. Conclusion ：tenuigenin have significantly potective effect on the PC12 cells injury induced by H2O2.
出处
《中药材》
CAS
CSCD
北大核心
2007年第8期991-993,共3页
Journal of Chinese Medicinal Materials
基金
广东省植物发育工程重点实验室开放基金(200405)
