摘要
目的:探讨中药半枝莲黄酮类化合物A06对体外肿瘤血管生成的影响.方法:分别采用小管形成实验、Transwell小室、酶联免疫吸附测定法(ELISA)、硝酸还原酶法检测半枝莲黄酮类化合物A06对人脐静脉内皮细胞(HUVEC)小管形成、迁移及人宫颈癌Hela细胞血管内皮细胞生长因子(VEGF)、一氧化氮(NO)表达的影响.结果:与对照组相比,半枝莲黄酮类化合物A06(50和100 mg/L)都能有效抑制HUVEC细胞的迁移(37.7%±10.7%,13.7%±6.0%vs 68.0%±8.2%,均P<0.01),减少小管样结构形成(9.7%±4.5%,1.8%±1.2%vs 30.2%±5.4%,均P<0.05),以及降低24 h后Hela细胞VEGF和NO的表达(VEGF:135.6±14.6 ng/L,108.3±7.7 ng/L vs 231.1±6.4 ng/L,均P<0.01;NO:42.3±2.3μmol/L,25.9±5.2μmol/L vs 70.1±8.1μmol/L,均P<0.01).结论:半枝莲黄酮类化合物A06有抑制体外肿瘤血管生成的作用,可能与抑制肿瘤细胞血管生成相关因子的表达,抑制内皮细胞迁移、形成管样结构有关.
AIM： To explore the effects of the extract flavonoid A06 from Scutellaria barbatae D.DON on tumor angiogenesis in vitro. METHODS： Human umbilical venous endothelial cells （HUVECs） were treated with different concentrations of the flavonoid A06 （25, 50 and 100 mg/L）. The effect of flavonoid A06 on the formation and migration of tubules in cultured HUVECs was evaluated by tube formation assay and Transwell chambers. The effect of flavonoid A06 on protein expression of vascular endothelial growth factor （VEGF） and nitric oxide （NO） in human cervical carcinoma Hela cells was detected by enzyme-linked immunosorbent assay and the nitrate reductase method. RESULTS： Flavonoid A06 extract from Scutellaria barbatae D.DON at two concentrations （50 and 100 mg/L） effectively inhibited migration （37.7% ± 10.7%, 13.7% ± 6.0% vs 68.0% ± 8.2%, both P 〈 0.01） and tubule formation in HUVECs （9.7%± 4.5%, 1.8% ± 1.2% vs 30.2% ± 5.4%, both P 〈 0.05） after 24 h. Flavonoid A06 also decreased the protein expression of VEGF and NO in Hela cells after 24 h （VEGF, 135.6 ± 14.6 rig/L, 108.3 ± 7.7 ng/L vs 231.1 ± 6.4 ng/L, both P 〈 0.01; NO, 42.3 ± 2.3 μmol/L, 25.9 ± 5.2 μmol/L vs 70.1 ± 8.1μmol/L, both P 〈 0.01）. CONCLUSION： Flavonoid A06 inhibits tumor angiogenesis in vitro. Its mechanism may be related to the inhibition of the expression of VEGF and NO, endothelial cell migration and tubule formation.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第20期2215-2219,共5页
World Chinese Journal of Digestology
基金
江苏省自然科学基金
No.BK2001149
