摘要
目的:探讨HBV-DNA荧光定量、乙肝前S1抗原(Pre-S1)与HBV血清学标志物(HBVm)之间的相关性。方法:采用荧光定量PCR、酶联免疫吸附法(ELISA)对290份HBsAg阳性标本进行HBV-DNA、PreS1、HBVm检测。结果:在290例检测标本中,HBeAg阳性共119例,其中HBV-DNA荧光定量阳性111例,阳性率93.3%(111/119);Pre-S1阳性101例,阳性率84.9%(101/119)。HBeAg阴性共171例,其中HBV-DNA荧光定量阳性74例,阳性率43.3%(74/171);Pre-S1阳性70例,阳性率41.2%(70/171)。HBeAg阴性171例中,Pre-S1存在于抗HBe阳性血清(119)中61例,阳性率51.26%(61/119);Pre-S1存在于抗HBe阴性血清(52)中9例,阳性率17.3%(9/52)。HBV-DNA阳性在抗HBe(+)血清(119)中占63例,阳性率52.9%(63/119);在抗HBe阴性血清(44)中占14例,占31.8%(14/44)。结论:HBV-DNA、Pre-S1可作为HBV感染的早期诊断指标,对乙型肝炎诊断、疗效判断具有十分重要的意义。
Objective：To study the associations between HBV-DNA and Pre-Sl antigen and serum markers of HBV. Methods： Pre-S1 antigen, immunological markers and HBV-DNA Concentrations of 290 sera of patients with HBsAg were detected by the ELISA （enzyme linked immunosorbent assay） and FQ-PCIL Results ：The positive rates of HBV-DNA and Pre-S1 antigen were respectively 93.3% （111/119） and 84. 9% （101/119） among HBeAg positive 119 patients. In HBeAg negative 171 patients, the positive rates of HBV-DNA and Pre-S1 antigen were respectively 43.3% （74/171）, 41.2% （70/171）. Pre-S1 antigen were 51.26% （61/119） in anti-HBe positive and HBeAg negative patients. Pre-S1 antigen were 17. 3% （9/52） in anti-HBe negative and HBeAg negative patients. HBV-DNA were 52. 9% （63/119 ） in anti-HBe positive and HBeAg negative patients. HBV-DNA were 31.8% （ 14/44 ） in anti-HBe negative and HBeAg negative patients. Conclusion ：The amont detection of Pre-S1 antigen and HBV DNA can be used as a helpful tool for the earlier diagnosis of HBV infection, and their clinical evaluation.
出处
《中国卫生检验杂志》
CAS
2007年第8期1484-1485,共2页
Chinese Journal of Health Laboratory Technology
