摘要
采用反转录聚合酶链反应（ＲＴＰＣＲ）从南京地区某输血后丙型肝炎患者血清中克隆出庚型肝炎病毒（ＨＧＶ）非结构区部分基因。序列分析结果表明：该序列与国外发表的庚型肝炎病毒ＨＧＵ４４４０２，ＨＧＵ４５９６６，ＨＧＵ３６３８０（ＧＢＶＣ）对应位置核苷酸序列同源性分别为８９０９％，９２１２％，８７２７％，与已报道的ＨＧＶ河北株序列同源性为９３９４％。对４０份输血后丙型肝炎血清和３０份非甲非乙非丙非丁非戊型肝炎血清进行了检测，ＨＧＶＲＮＡ阳性率分别为１０００％和６６７％。
Partial gene of hepatitis G virus(HGV) was cloned and sequenced from the serum of a patient with post-transfusion hepatitis C in Nanjing of China by reverse transcription-polymerase chain reaction(RT-PCR). The sequence showed 89 09%, 92 12%, 87 27%,93 94% nucleotide identity over the corresponding region of HGU44402, HGU45966, HGU36380 in America and HGV isolate in Hebei Province of China respectively. Forty patients with post-transfusion hepatitis C and thirty patients with hepatitis non A-E were detected for HGV RNA by RT-PCR, the HGV RNA positive rate were 10 00% and 6 67% respectively.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1997年第1期27-28,共2页
Chinese Journal of Experimental and Clinical Virology
