摘要
目的进一步了解燃煤砷污染对人体免疫功能的远期影响,为燃煤型砷中毒的预防和干预提供科学依据。方法采用碱性磷酸酶抗碱性磷酸酶桥联酶标法(APAAP)测定燃煤型砷中毒患者外周T淋巴细胞亚群CD3+、CD4+、CD8+细胞的变异情况,并比较1998年与2005年各项指标的变化。免疫比浊法测定患者血清中免疫球蛋白IgGI、gMI、gM及补体C3、C4的含量。结果燃煤型砷中毒患者T淋巴细胞亚群CD3+、CD4+细胞和CD4+/CD8+比值显著低对照组(P<0.01),1998年与2005年比较差异无统计学意义(P>0.05);患者血清中IgG和C4分别显著高于和低于对照组(P<0.01),其余指标差异无统计学意义(P>0.05)。结论燃煤砷污染所致人体免疫球蛋白及补体改变以及T淋巴细胞亚群免疫功能的持久性损害,不仅可能是砷中毒的促发原因之一,还可能是该病病情持续发展的原因之一,采取针对性提高免疫功能的防治策略有助于砷中毒的预防和干预。
Objective To explore further the long-term effects on human immune function of arsenic exposure caused by burning coal, and to provide scientific evidences for prevention and intervention of arsenism. Methods Variation conditions of peripheral blood T - lymphocyte subsets Cd3^＋, CD4^＋ and CD8^＋ cells were measured with alkaline phosphatase - anti - alkaline phosphatase bridged enzyme linked immunosorbent assay （APAAP）, and analyse the changes of T - lymphocyte subsets before and after 7 years. The contents of serum immunoglobnlin IgG, IgM and IgA, and complement C3, CA were detected with immune turbidimertry. Results The content of IgG in arsenism patiens was significantly higher than the control group, while the level of CA, C3^ ＋, CD4^＋ and CD4^＋/CD8^＋ were significantly decreased compared with control group （ P 〈 0.05）, The differences of other indexes did not have significance in statistic. The levels of T-lymphocyte subsets didn＇t show evident changes before and after 7 years（P 〈 0.05）, Conclusion The changes of human immunoglobulin and complement, and persistent of T - lymphocyte subsets might not only be a cause of arsenism, but also be related with the continual developing condition of arsenism. Taking some pointed measures to enhance immunological function may be helpful for the prevention and intervention of arsenism.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第9期1106-1107,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30460123)
贵州省重大专项基金[黔科合重点大专项字(2006)6016]
贵州省"十一五"攻关项目[2006]贵州省省长基金
关键词
燃煤砷污染
人体
免疫球蛋白
补体
T淋巴细胞胞亚群
arsenic pollution caused by burning coal
human
immunoglobulin
complement
T-lymphocyte
