摘要
以橙色叶球大白菜为试材对总黄酮提取及含量测定方法进行了研究。通过单因素试验和L12(43)正交试验筛选出最佳提取方法是超声波法,其提取工艺是以80%乙醇为溶剂,在料液比(g/mL)为1∶20,温度60℃条件下,用频率为60Hz的超声波连续提取2次,每次提取1h。以芦丁为标样,利用分光光度法和RP-HPLC法进行黄酮测定,比较表明,分光光度法和RP-HPLC法测定的结果趋势一致,RP-HPLC法优于分光光度法。建立了大白菜黄酮的RP-HPLC测定法,流动相为甲醇∶水∶乙酸=6∶93.4∶0.6,流速0.5mL/min,柱温25℃,检测波长280nm。
The methods of extraction and determination of the total flavonoids in the leaf-head of heading Chinese cabbages [ Brassica campestris ssp. pekirtensis （ Lour. ） Olsson ] were studied. The optimal conditions for the ultrasonic extracting process were screened by single factor analysis and orthogonal analysis, which using 80% ethanol as impregnant, at the ratio 1 ： 20 of solid to liquid （g/mL） , the total flavonoids were extracted by 60 Hz ultrasonic at temperature 60℃ for 1 h, 2 times. Using rutin as standard compound, the total flavonoids of orange colour Chinese cabbages were determined by UV spectrophotometry and RP-HPLC, respectively. The result shows that RP-HPLC is better than spectrophotometry with the same trend. The condition of RP-HPLC is that a ODS column was used with methanol-water-acetic acid （6：93.4 ： 0.6 ） as the mobile phase, at a flow rate of 0.5 mL/min, 25 ℃ of the column temperature and 280 nm of the UV detectection wavelength.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第4期923-928,共6页
Acta Horticulturae Sinica
基金
国家‘863’项目(2003AA207120)
陕西省科技攻关项目
