摘要
应用锚定ISSR技术对21个栽培黑木耳（Auricularia auricula）菌株进行了遗传多样性研究。从20个引物中选出10个ISSR引物，扩增得到185个扩增位点，大小分布在200-3000bp之间，其中多态性位点181个，多态性位点占97.84%，表明ISSR标记的多态性非常高。平均每个位点的观察等位基因数（Na）为1.9784，每个位点的有效等位基因数（Ne）为1.2396；菌株间平均Nei基因多样性（h）为0.2732，Shannon信息指数（I）为0.4278。Nei（1972）遗传距离矩阵分析表明21个黑木耳菌株间遗传距离在0.36到0.55之间，基因流（Nm）达到2.7528，表明我国栽培黑木耳的遗传背景十分丰富，遗传多样性很高，同时表明我国各地域间栽培黑木耳菌株存在着很大的菌种交流。
Genetic diversity was assessed in 21 cultivated strains of A. auricula by ISSR （inter simple sequence repeats）. Ten primers were screened out from twenty ISSR primers in this study. One hundred and eighty five loci were produced from the primers, of which 181 loci （97.84%） were polymorphic in the organism. The size of these loci ranged from 200 bp to 3 000 bp. High genetic diversity of A. auricula in China was detected, the observed number of alleles （Na） averaged 1. 9784 and the effective number of alleles （Ne） was 1. 2396, for every locus ; Nei＇s genetic diversity （h） was estimated to be 0. 2732, while Shannon＇s information index （I） was 0. 4278, on average. The Nei＇s （1972） genetic distance among strains ranged from 0. 36 to 0.55, but the gene flow （Nm） was 2. 7528. Analysis showed that there were high genetic diversity and large spawns exchange among provinces for A. auricula in China.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第4期935-940,共6页
Acta Horticulturae Sinica
基金
科技部资助项目(2004EP090020)
