摘要
S-c是控制水稻籼粳亚种间杂种F1花粉不育性的基因座位之一。在该座位,台中65的基因型为Sj/Sj,广陆矮4号的基因型为Si/Si。以台中65和广陆矮4号为遗传测验种,分别与4个粳型亲籼系配组,根据部分F2群体中植株花粉育性表型及与S-c紧密连锁分子标记基因型的偏态分离程度,测定了这4个粳型亲籼系在S-c座位的基因型,结果表明,G2416-3的基因型为Si-2/Si-2;G2605和G3004-4的基因型均为Si-1/Si-1;G2417-2-1的基因型为Sn/Sn。本文还对F1花粉不育性基因遗传分化的测定方法进行了讨论。
S-c is one of the F1 pollen sterility loci between indica and japonica cultivars in rice. The genotype of Taichong 65(T65) and Guangluai 4(GLA 4) at the locus is S^j/S^j and S^i/S^i, respectively. Four indica-compatiblejaponica lines(ICJLs),G2416-3,G2417-2-1 ,G2605 and G3004-4 were crossed with testers,T65 and GLA 4,and their S-c locus genotypes were identified. The segregation of PCR-based molecular markers linked tightly with S-c locus and plant pollen fertility in several F2 populations showed that G2416-3 possessed S^i-2/S^i-2 genotype; G2605 and G3004-4 possessed S^i - 1/S^i- 1 genotype; while G2417-2-1 possessed S^n/S^n genotype. The test methods on genetic differentiation of the F1 pollen sterility loci were also discussed in this paper.
出处
《华北农学报》
CSCD
北大核心
2007年第4期5-8,共4页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金(30471068)
广东省科学技术项目资助(2004B20101003)
关键词
水稻
粳型亲籼系
花粉不育性
复等位基因
微卫星标记
Rice( Oryza sativa L. )
Indica-compatible japonica lines
Pollen sterility
Multi-alleles
Microsatellite marker