摘要
应用超声提取、固相萃取(SPE)、薄层色谱(TLC)技术,成功提取、分离了海水悬浮颗粒物中的色素类化合物,并对叶绿素a(Chl a)的碳同位素进行了测定,建立了海水悬浮颗粒物中叶绿素组分的TLC分离及稳定碳同位素测定方法.测定了长江口外冲淡水区颗粒有机碳(POC)和Chl a中的碳同位素.发现悬浮颗粒物中Chl a的δ13C介于-28.17‰～-32.29‰之间,平均值-29.75‰;POC的δ13C介于-17.92‰～-23.19‰之间,平均值-20.67‰,Chl a比POC的δ13C偏负9.08‰.表层Chl a的δ13C值由北向南逐渐偏负,由近岸向开阔海域逐渐偏正.
Pigment compounds in suspended particulate matter （SPM） collected from Changjiang Estuary plume have been extracted and separated successfully using supersonic extraction,silicate cartridge and thin layer chromatography （TLC） techniques. Stable carbon isotope ratio analysis for single chlorophyll a and particulate organic carbon （POC） were established using the elemental analysis-isotope ratio mass spectrometry （EA-IRMS）. The results showed that δ13C of chlorophyll a ranged from -28.17‰ to -32.29‰ with an average of -29.75‰, and that δ13C of POC ranged between -17. 92‰ and -23. 19‰ with an average of -20. 67‰. The average of δ13C of chlorophyll a was 9.08‰ lighter than that of POC. The distribution of δ13 C Chl a（‰） for surface water samples showed decrease tendency from north to south and increase tendency from the shore to open sea in the estuary.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2007年第A01期49-53,共5页
Journal of Xiamen University：Natural Science
基金
厦门大学"海洋学生科研奖励计划"项目
厦门大学科技创新项目(XDKJCX20051007)资助
关键词
悬浮颗粒物
叶绿素A
碳同位素
薄层色谱
长江口
suspended particulate matter
chlorophyll a
carbon isotope thin layer chromatography
Changjiang Estuary
