摘要
目的探讨不同方法制备的生物衍生骨植入大耳白兔体内后的组织学变化,为组织工程研究提供异种动物源支架材料。方法取新鲜市售猪股骨两端,除去软组织及骨膜后切割为0.5cm×0.5cm×0.5cm大小骨块。根据不同处理方法分为5组:A组仅用生理盐水冲洗,B组经脱脂处理,C组经脱脂脱钙处理,D组经脱脂脱钙脱细胞处理,E组经脱脂脱细胞处理。取各组骨块行微量元素检测;经25kGy辐照灭菌后植入26只新西兰大耳白兔股部肌袋内,左侧植入A、B组骨块,右侧植入C、D及E组骨块,各肌袋植入1块。术后2、6及12周取材,作HE及Masson染色,行炎性细胞、破骨细胞计数,观察胶原纤维生成及成骨情况。结果各组骨块微量元素残留量符合标准。所有动物术后生存良好。各组实验兔植入部位均未见组织坏死、积液及化脓感染。炎性细胞计数以A组为最多,与其他各组比较差异有统计学意义(P<0.05);破骨细胞计数各组间差异无统计学意义(P>0.05)。HE及Masson染色观察,胶原纤维及新骨形成C、D组为最好,E组次之,A、B组最差。结论脱脂脱钙脱细胞处理的猪股骨有良好的降解性、成骨性及较低的炎性反应,有可能成为组织工程骨的支架材料。
Objective To explore the histological changes of bio-derived bone prepared by different methods after implantation, and to provide the scaffold material from xenogeneic animal for tissue engineering. Methods The extremities of porcine femur were cut into 0.5 cm × 0. 5 cm × 0. 5 cm. Then they were divided into 5 groups according to different preparation methods ： group A was fresh bone just repeatedly rinsed by saline ; group B was degreased ; group C was degreased and decalcificated; group D was degreased, acellular and decalcificated ; group E was degreased and acellular. All the materials were implantated into femoral muscle pouch of rabbit after 25 kGy irradiation sterilization. The cell counting of inflammatory cells and osteoclasts, HE and Masson staining, material degradation, collagen and new bone formation were observed at 2, 6, and 12 weeks postoperatively. Results The residue level of trace element in biomaterials prepared by different methods is in line with the standards. All the animals survived well. There were no tissue necrosis, fluid accumulation or inflammation at all implantation sites at each time point. The inflammatory cells counting was most in group A, and there was significant difference compared with other groups（P〈0. 05）. There was no significant difference in osteoclasts counting among all groups. For the index of HE and Masson staining, collagen and new bone formation, groups C and D were best, group E was better, and groups A and B were worse. Conclusion The degreased, acellular and decalcificated porcine bone is better in degradation, bone formation, and lower inflammatory reaction, it can be used better scaffold material for tissue engineered bone.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2007年第9期1003-1006,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家高技术研究发展计划(863)资助项目(2001AA216011)
北京科学技术委员会科技计划重大资助项目(H060920050730)~~
关键词
组织工程骨
猪源性材料
组织相容性
兔
Tissue engineered bone Porcine material Histocompatibility Rabbit
