摘要
建立了高效液相色谱法测定培福新注射液中培氟沙星和维生素Ｃ钠的含量，采用Ｃ１８柱，１０μｍ（大连化物所Ｓｐｈｅｒｉｓｏｒｂ），以乙腈－柠檬酸液（０．０５ｍｏｌ／Ｌ）－醋酸铵液（０．５ｍｏｌ／Ｌ）－１％磷酸水溶液（２２∶７５∶１∶２，内含０．１％三乙胺）为流动相，在紫外检测器２５４ｎｍ波长处进行检测。培氟沙星线性范围６０～２４０μｇ／ｍｌ，ｒ＝０．９９９７（ｎ＝５），维生素Ｃ钠线性范围１～９μｇ／ｍｌ，ｒ＝０．９９９９（ｎ＝５）。方法简便，结果满意。
This paper described the determination of pefloxacin and sodium ascorbate in peflacine by HPLC C 18 (10μm) was used as the column The mobile phase consisted of a mixture of acetonitrile citric (0 05mol/L) ammonium acetate (0 5mol/L) phosphoric acid (1%) (22∶75∶1∶2,containing 0 1% triethylamine) The wavelength of determination was 254nm The results showed that the linear range of pefloxacin and sodium ascorbate were 60￣240 μg/ml (r=0 9997,n=5) and 1￣9μg/ml (r=0 9999,n=5) respectively This method is accurate,simple and rapid
出处
《广东药学院学报》
CAS
1997年第1期18-20,共3页
Academic Journal of Guangdong College of Pharmacy
