摘要
目的评估图像细胞仪（ＩＣＭ）所观察的几项参数用以揭示肝细胞癌（ＨＣＣ）形成门静脉癌栓（ＴＴＰＶ）的价值。方法９９例ＨＣＣ以ＣＡＳ┐２００型ＩＣＭ行ＤＮＡ定量分析。结果ＤＮＡ指数（ＤＩ）和增殖指数（ＰＩ）在合并ＴＴＰＶ的ＨＣＣ分别显著高于无ＴＴＰＶ的ＨＣＣ（Ｐ＜０．０５，Ｐ＜０．０１）。在同为二倍体的ＨＣＣ中，合并ＴＴＰＶ者ＰＩ值显著高于无ＴＴＰＶ者（Ｐ＜０．０５）。合并ＴＴＰＶ的ＨＣＣ，二倍体肿瘤的比例低于无ＴＴＰＶ者，而异倍体比例、细胞核面积、超５倍体细胞比例均高于无ＴＴＰＶ的ＨＣＣ，但Ｐ＞０．０５。结论随着ＤＮＡ合成水平的上升，ＨＣＣ细胞获得了侵袭门静脉、形成ＴＴＰＶ的能力；以ＩＣＭ行ＤＮＡ定量分析对揭示ＨＣＣ是否具有较强侵袭性有明显价值，其中ＰＩ是较为敏感的参数。
Objective:Value of image cytometric parameters for predicting tumor thrombogenesis in portal vein (TTPV) in hepatocellular carcinoma (HCC)was evaluated. Methods:DNA content in 99 resected specimens of human HCC was analysed with CAS 200 image cytometry. (1)39 specimens had macroscopic TTPV,(2)30 had microscopic TTPV,and (3)30 were free of TTPV. Results:DNA index and proliferating index (PI) were significantly higher in (1)and (2) than in (3) ( P <0 05, P <0 01) In diploid HCC,PI was markedly higher in (1) and (2) than (3) ( P <0 01). Diploid rate was lower,aneuploid rate,nuclear area and super pentaploid rate were higher in (1) and (2) Conclusions:HCC cells obtained stronger invasive and metastatic capability once DNA prolifeating process increased. The DNA image cytometry has noticeable value in revealing the potency of invasiveness in HCC.
出处
《肿瘤》
CAS
CSCD
北大核心
1997年第3期145-147,共3页
Tumor
