摘要
利用猪圆环病毒2型(PCV2)特异性引物,对吉林省某猪场发生的具有典型断奶仔猪多系统衰竭综合征(PMWS)症状病猪的淋巴结、脾脏组织进行PCR检测。将检测阳性的病料研磨、高心、过滤除菌,接种到无PCV污染的PK-15细胞进行病毒分离。将PK-15细胞增殖所得的病毒纯化后,在电镜下观察到直径约为17 nm的圆形病毒样粒子。PCV2间接免疫荧光试验,可在细胞浆中观察到特异性荧光颗粒。PCV2特异性PCR检测细胞培养物,获得阳性结果。由此说明,分离出的病毒为猪圆环病毒2型。
Lymph nodes and spleen of a swine with typical syndrome of PMWS broken out in one porcine farm in Jilin province were detected by polymerase chain reaction（PCR） with a pair of primers specific to porcine circovirus type 2（PCV2） The PCV2 PCR positive samples were abraded, sedimented by centrifugation, filtered and inoculated to the PK-15 cell line, which were not contaminated by PCV1. The viral particles were purified and observed with electronic microscope. They were 17nm in diameter. In the cytoplasm, the specific fluorescence particles were observed in the frozen section stained by indirect immunofluorescence test specific to PCV2. And the specific nucleotide was amplified from the cell culture by the PCR, too. According to the above results, the isolated strain belongs to PCV2.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2007年第4期433-436,共4页
Journal of Jilin Agricultural University
基金
吉林省牧业局资助项目(20D40620)
吉林农业大学博士启动基金(20050420)
