摘要
To support the scientific basis for rapid identification of pathogenic bacteria and other stud- ies,the sequences of hsp60 gene in major 34 species of 16 genus of pathogenic bacteria were search out in GenBank and a proper pair of universal degenerate primer was designed by means of the molecu- lar biological softwawe Primer 5.0 and Oligo 6.0.This primer was then used in the PCR amplification, and the hsp60 gene fragments of the selected pathogenic bacteria could be amplified using this degener- ate primer.By way of bioinformational analysis,the conservation,variation and the interspecies phylo- genetic relations of the hsp60 gene sequence were analysed.From the results of the comparative study on sequences,it was demonstrated that the hsp60 gene was characterized by conservation and varia- tion,in which the conserved and mutant regions co-existed and separately distributed with many small mutant regions distributed among the conserved regions,just like the mosaic.The phylogenetic tree among different pathogenic bacteria drawn from the hsp60 gene analysis was proved to be consistent with those from 16S rRNA and 23S rRNA.It is concluded that the sequence distribution of hsp60 gene would provide a solid basis for the rapid identification of pathogenic bacteria and the development of a diagnostic microarray.
To support the scientific basis for rapid identification of pathogenic bacteria and other studies, the sequences of hsp60 gene in major 34 species of 16 genus of pathogenic bacteria were search out in GenBank and a proper pair of universal degenerate primer was designed by means of the molecular biological softwawe Primer 5.0 and Oligo 6.0. This primer was then used in the PCR amplification, and the hsp60 gene fragments of the selected pathogenic bacteria could be amplified using this degenerate primer. By way of bioinformational analysis, the conservation, variation and the interspecies phylogenetic relations of the hsp60 gene sequence were analysed. From the results of the comparative study on sequences, it was demonstrated that the hsp60 gene was characterized by conservation and variation, in which the conserved and mutant regions co-existed and separately distributed with many small mutant regions distributed among the conserved regions, just like the mosaic. The phylogenetic tree among different pathogenic bacteria drawn from the hsp60 gene analysis was proved to be consistent with those from 16S rRNA and 23S rRNA. It is concluded that the sequence distribution of hsp60 gene would provide a solid basis for the rapid identification of pathogenic bacteria and the development of a diagnostic microarray.
