摘要
目的:观察枸杞多糖对皮肤胶原代谢和自由基产生的影响,探讨其抗皮肤衰老的作用。方法:实验于2005-06/2006-05在广东医学院整形外科研究所完成。①实验材料:清洁级昆明小鼠60只,月龄2个月,体质量16～24g,雌雄各半。②实验分组:将小鼠随机分为正常对照组、衰老模型组和抗衰老模型组,每组20只。③实验干预:模型组每日用D-半乳糖溶液皮下注射制造衰老模型,用量和时间为80mg/(kg·d)7d,120mg/(kg·d)14d,140mg/(kg·d)14d,180mg/(kg·d)7d。正常对照组每日注射同体积的生理盐水。抗衰老模型组在注射D-半乳糖期间以枸杞多糖灌胃,剂量为20mg/(kg·d),正常对照组和衰老组则以同体积的生理盐水代之灌胃。④实验评估:42d后切取小鼠颈背部皮肤,测定超氧化物歧化酶活力、羟脯氨酸和丙二醛含量。结果:56只小鼠进入结果分析(4只死亡)。①小鼠皮肤超氧化物歧化酶活力:与正常对照组相比,衰老组和抗衰老组小鼠皮肤超氧化物歧化酶活力降低,差异有显著性意义(P<0.01);抗衰老组与衰老模型组比较,超氧化物歧化酶活力增加,差异有显著性意义(P<0.01)。②与正常对照组相比,衰老组和抗衰老组小鼠皮肤羟脯氨酸和丙二醛含量增加,差异有显著性意义(P<0.01);抗衰老组与衰老组比较,羟脯氨酸和丙二醛含量均降低,差异有显著性意义(P<0.01)。结论:枸杞多糖改善皮肤老化的作用与提高小鼠皮肤超氧化物歧化酶活力,降低羟脯氨酸、丙二醛含量,影响胶原代谢有关。
AIM： To investigate the effect of Lycium barbarum polysaccharides （LBP） on collagen metabolism, free radical production and anti-aging of skin. METHODS： The experiment was conducted in the Institute of Plastic Surgery of Guangdong Medical College from June 2005 to May 2006. ①Sixty clean-grade Kunming mice aged 2 months with the body mass of 16-24 g were selected, male and female （half and half）. ②All mica were randomly divided into three groups, normal control group, aging model group and anti-aging group with 20 in each group.③The aging mice of the aging model group were established by subcutaneous administration with a dose of D-galactose into mice. At beginning, the dose was 80 mg/kg per day for 7 days, to follow the dose was 120 mg/kg per day for 14 days, and subsequently 140 mg/kg per day for 14 days, and at last the dose was 180 mg/kg per day for 7 days, while the mice of normal control group were injected with the same volume of saline. In the meantime, the mice of the anti-aging group received LBP by intragastric administration with the dose of 20 mg/kg per day, while the mica of the normal control group and the aging model group received the same volume of saline by intragastric administration. ④After 42 days, activity of superoxide dismutase （SOD） and contents of hydroxyproline （Hyp） and malondialdehyde （MDA） in skins cut from the nape of mice were measured. RESULTS： Totally 56 mice were involved in the result analysis, because of 4 deaths. ①Activity of SOD in rat skins was lower in the anti-aging group and aging model group than the normal control group （P 〈 0.01 ）. The activity of SOD was higher in the anti-aging group than the aging model group （P 〈 0.01 ）. ②Contents of Hyp and MDA of rat skins were higher in the anti-aging group and aging model group than the normal control group（P 〈 0.01 ）. The contents of Hyp and MDA of rat skins were lower in the anti-aging group than the aging model group（P 〈 0.01 ）. CONCLUSION： The influence of LBP on delaying skin aging is associated with increasing the activity of SOD, decreasing the contents of Hyp and MDA in skins of mice and collagen metabolism.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第36期7207-7210,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
2005年度湛江市科技攻关项目(71)~~
