摘要
目的探讨人类白细胞抗原DRw53(HLA-DRw53)基因在变应性血管炎患者治疗前后mRNA表达量的变化。方法采用低渗盐析法从外周血中分离白细胞,Trizol试剂提取白细胞总RNA,1%甲醛变性琼脂糖凝胶电泳和紫外分光光度计检测其完整性和浓度。利用随机引物逆转录合成互补脱氧核糖核酸(cDNA),根据HLA-DRw53基因第二外显子序列和管家基因人磷酸甘油醛脱氢酶(GAPDH)的cDNA序列设计序列特异性引物。检测43例变应性血管炎患者治疗前后与24名正常人HLA-DRw53基因mRNA表达量的变化。结果治疗前组HLA-DRw53基因mR-NA表达与正常对照组和治愈组比较差异有统计意义(P<0.01);治愈组表达量与正常对照组比较差异无统计学意义(P>0.05)。结论HLA-DRw53基因与变应性血管炎之间存在相关性,且可能在抗原呈递中起作用,这对指导变应性血管炎的病因研究以及治疗方向有一定的意义。
Objective To observed the variations of quantity of human leucocyte antigen DRw53 gene （HLA - DRw53） expressed in allergic vasculaltis patients before and after treatment. Methods The total RNA of leucocyte was extracted 43 allergic vasculitis patients and 24 normal subjects before and after treatment with Trizol reagent and the integrity and concentrations of HLA - DRw53 gene mRNA were determined by agarose gel electrophoresis and UV spectrophotometer after denatured with 1% formaldehyde, the product was transcribed into complementary retroviral synthetic deoxyribonucleic acid （cDNA）, and sequence - specific primers were designed based on the sequences of second HLA - DRw53 gene exon and housekeeping gene glyceraldehyde phosphate - dehydrogenase （GAPDH） cDNA. Results The expression of HLA - DRw53 gene mRNA in the pretreatmetn group was signitieantly different as compared with that of the normal control group and cure group （P 〈 0.01）; There no significant differences were observed in the expression of HLA - DRw53 gene mRNA between the cured group and the normal control group （ P 〉 0.05）. Conclusion There is correlation between the expression of HLA - DRw53 gene and allergic vasculitis.
出处
《中国热带医学》
CAS
2007年第9期1542-1543,共2页
China Tropical Medicine
