摘要
目的测定健康正常Balb/C、C3H/HeJ和C3H/SW小鼠外周血淋巴细胞亚群含量,比较3种小鼠的差异性。方法用流式细胞仪测定Balb/C、C3H/HeJ和C3H/SW小鼠外周血淋巴细胞亚群含量。结果各20例3种小鼠、5种外周血淋巴细胞亚群含量呈正态分布,95%可信区间可以被认可;Balb/C与C3H/SW小鼠除B细胞外其他4种淋巴细胞亚群(T细胞、Ts细胞、Th细胞、NK细胞)含量差异性不大(P>0.05);而C3H/HeJ小鼠5种淋巴细胞亚群(T细胞、B细胞、Ts细胞、Th细胞、NK细胞)含量均比C3H/HeJ、C3H/SW小鼠低,并具有统计学意义(P<0.05)。结论建立了健康正常Balb/C、C3H/HeJ和C3H/SW小鼠5种外周血淋巴细胞亚群含量正常参考值;C3H/HeJ小鼠细胞免疫功能比Balb/C、C3H/SW小鼠低,易受细菌等侵染。
Objective To establish and compare the reference range of normal values of five types of lymphocyte subsets in mice of three strains by flow cytometry. Method The five types of Th1/Th2 cytokines in Balb/C, C3H/HeJ and C3H/SW mice were determined by flow cytometry. The results were statistically analyzed with SPSS 10.0 software. Resoults The distribution of the five lymphocyte subsets presents a Gaussian distribution in the 3 mouse strains, respectively. The 95% confidence interval for mean values can be accepted. The difference at lymphocyte subsets level is not significant in Balb/C and C3H/SW mice except B cells. The values of lymphocyte subsets of C3H/HeJ mice were lowest among the three strains of mice. Conclusion The normal reference values of Th1/Th2 cytokine in Balb/C, C3H/HeJ and C3H/SW mice have been established. The cellular immune function of C3H/HeJ is most feeble among the three strains, and C3H/HeJ mice may be susceptible to infection with pathogenic microorganisms.
出处
《中国比较医学杂志》
CAS
2007年第8期I0012-I0015,共4页
Chinese Journal of Comparative Medicine
基金
辽宁省科技资助项目(2006408002-4)
辽宁省重点实验室重点资助项目。
关键词
流式细胞术
淋巴细胞亚群
正常参考范围
Flow cytometry
Lymphocyte sbsets
Reference ranges, normal values
Mice
