摘要
利用农杆菌介导的转基因技术,成功地将通过RT-PCR克隆的拟南芥ICE1基因导入垦鉴稻10号中,经PCR和Southern检测确认目的基因已整合到水稻基因组中。潮霉素抗性测定结果表明,与未转基因水稻相比,T1代表现出对潮霉素较高的抗性和孟德尔式的单位点遗传。抗寒能力检测结果表明,在同等低温胁迫条件下T1代转基因株系的死亡率明显低于未转基因对照。脯氨酸含量增幅明显高于未转基因对照。上述结果表明,转ICE1水稻提高了抗寒能力。
An Arabidopsis thaliana transcriptional factor gene ICE1 cloned by RT-PCR was successfully transferred into rice variety Kenjiandao 10 by Agrobacterium, mediated transgenic technique. PCR amplification and Southern blot analysis indicated that ICE1 had been integrated into rice genome. Compared with non transgenic plants, transgenic lines exhibited high resistance to hygromycin B and inherited in Mendelian single-copy. Under the same low temperature stress conditions, transgenic plants had lower mortality rate and higher increasing range of proline contents compared with non-transgenic plants, suggesting that cold tolerance of transgenic rice plants with ICEI gene was improved.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2007年第5期482-486,共5页
Chinese Journal of Rice Science
基金
黑龙江省教育厅科学技术研究项目(11511248)
关键词
ICE1基因
水稻
遗传转化
农杆菌介导
耐寒性
脯氨酸含量
ICE1 gene
rice
genetic transformation
Agrobacterium-mediated method
cold tolerance
proline content
