摘要
目的用基因工程方法获得屎肠球菌类透明质酸酶(Hyalronidase,Hyl)蛋白,经口服免疫小鼠后,探讨机体产生的免疫应答和抗感染保护作用。方法Hyl用Ni2+-NTA柱纯化后,作为抗原给小鼠灌胃进行免疫,ELISA检测小鼠血清IgG、IgA、小肠粘液sIgA和粪便sIgA。并用屎肠球菌TX0016腹腔攻击已免疫的小鼠,观察其免疫保护性。结果重组蛋白经Ni2+-NTA柱纯化后,灌胃免疫小鼠后。ELISA检测结果血清IgA、IgG,粪sIgA,肠粘液sIgA明显高于对照组(P<0.01)。通过细菌攻击后,小鼠的平均存活时间也明显长于对照组。结论重组蛋白Hyl经口服免疫可有效诱导粘膜免疫应答,产生高水平的sIgA,发挥局部粘膜免疫作用和抗菌保护作用。Hyl有望作为预防屎肠球菌口服疫苗的候选抗原。
To obtain the recombinant hyaluronidase（Hyl） protein of E. faefium by gene-engineering, the immune response in mice orally fed with Hyl protein and the immunoprotection of anti-infection immunity were investigated. The recombinant protein was purified by Ni^2＋ -NTA agarose and fed to mice by oral immunization. The change of antigen-specific antibody in the serum, mucosal fluid and faces were detected by ELISA assay, and the protective role of anti-bacterial immunity was evaluated after infection with E. faecium TX0016. The results showed that antigen-specific antibody was higher than controls as demonstrated by ELISA, and the average survival time was longer than controls too, after infection with TX0016. It concludes that the Hyl protein can induce effective mucosal immune response and produce higher levels of slgA after oral immunization with Hyl protein. The recombinant fusion protein Hyl can be used as an oral vaccine antigen for prevention of E. faecium infection.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2007年第9期913-915,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金资助项目(30475995)
关键词
屎肠球菌
透明质酸酶
口服免疫
特异性抗体
Enterococcus faecium
Hyaluronidase
oral immunization
specific antibody
