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相对定量的2^(-△△CT)法分析HLA-G基因在子宫内膜异位症中的表达 被引量:16

Relative quantitative detection of HLA-G mRNA expression in patients with endometriosis by 2^(-△△CT) Method
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摘要 目的检测人类白细胞抗原-G(HLA-G)在子宫内膜异位症(EM)在位与异位内膜组织中的表达。方法选取卵巢子宫内膜异位囊肿患者30例(实验组),非子宫内膜异位症患者30例(对照组),应用实时荧光定量RT-PCR技术检测实验组在位与异位内膜组织,及对照组在位子宫内膜组织中HLA-G mRNA的表达,2-△△CT法分析结果。结果HLA-G mRNA在实验组在位与异位内膜组织中的表达均明显高于对照组(P<0.05),两者比值约为3.5/1。实验组在位与异位内膜组织中的表达无明显差异(P>0.05)。结论HLA-G mRNA的表达异常可能与EM发病有关。 Objective: To investigate Human leukocyte antigen- G (HLA- G) mRNA expression in the endometrium of endometfiosis (EM). Methods: 30 women with ovarian endometriosis (OEM), 30 women without EM were recruited as study grou and control group, respectively. FQ - RT PCR was used for quantitative detection of the difference on HLA - G mRNA expression in the endometrium between two groups. 2^-△△CT method was used for analyzing the difference on HLA - G mRNA expression. Results: HLA - G mRNA expression of OEM group was significantly higher than that in the control group, the ratio is 3. 5/1. There was no obvious correlation between the expression of OEM and adenomycsis (AM). Conclusion: Higher HLA - G mRNA may be related to the pathomechanism of EM.
作者 柳玲
出处 《中国优生与遗传杂志》 2007年第9期25-26,共2页 Chinese Journal of Birth Health & Heredity
关键词 子宫内膜异位症 人类白细胞相关抗原-G 荧光定量PCR Endometriosis Human leukocyte antigen - G FQ - PCR
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  • 1Gazvani R, Templeton A, New considerations for the pathogenesis of endometriosis[J]. Int J Gynaecol Obstet, 2002,76(2) :117.
  • 2Livak KJ,Schmittgen TD. Analysis of relative gene expression data using real- time quantitative PCR and the 2 ( -Delta Delta C(T)) Method[J]. Methods,2001,25(4) : 402 -408.
  • 3Petrcff MG, Sedl mayr P, Azzola D, et al. Decidual macrophages are potentially susceptible to inhibition by class I a and I b HLA molecules[J]. J Reprod Immunol, 2002,56(1 -2) :3.
  • 4Ritean B, Rouas Freiss N, Menier C, et al. HLA - (;2, - G3, and G4 isoforms expressed as nonmature cell surface glycoproteins inhibit NK and antigen - specific CTL cytolysis[ J]. J Immunol,2001,166 (8) : 5018 -5026.

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