摘要
目的:探讨古方地黄饮子对β淀粉样蛋白(Aβ25-35)所致PC12细胞损伤时凋亡基因bax、bcl-2和caspase-3表达的调控作用。方法:把离体培养的进入对数生长期的PC12细胞分为6组:空白对照组、模型对照组、VitE脑脊液组、地黄饮子脑脊液低、中、高剂量组。待24h细胞贴壁后吸去培养液,分别加入空白培养液、正常脑脊液、VitE脑脊液、中药脑脊液低、中、高剂量,加培养液补至等量,37℃孵育2h。然后除空白组之外的各组加入经老化处理的Aβ25-35(终浓度为10μmol/L),建立AD细胞模型。空白组加入等量的培养液,继续孵育24h后离心收集细胞,提取总RNA。应用RT-PCR二步法和琼脂糖凝胶电泳方法测定bax、bcl-2和caspase-3基因的表达。结果:地黄饮子能下调bax、caspase-3基因的表达,对bcl-2基因的表达有上调作用。结论:地黄饮子脑脊液能有效降低bax、caspase-3基因表达,提高bcl-2的表达,从而抑制、延迟细胞凋亡过程,阻止细胞凋亡,促进PC12细胞存活。
Objective ：To investigate repressive effects on the expression of Apoptosis Gene bax, bcl -2 And caspase - 3 of Cells PC12 injury by 13 - amyloid 25 - 35 protein（ AI325 - 35 ）. Methods： Cultured Cells pc - 12 were divided into 6 groups.normal group, model group, vitamin E group,low dose of TCM group, moderate dose of TCM group and high dose of TCM group. Six groups were respectively added to normal cultured fluid, nomal cerebrospinal fluid, vitE cerebrospinal fluid,low dose of TCM cerebraspinal fluid,moderate and high dose of TCM cerebrospinal fluid in log growth phase, then added cultured fluid to equal,37℃hatched 2 hours. The normal group was added to the same dose of cultured fluid and other groups were added to Aβ25 -35 dealed with aging （the final concentration is 10μmol/L）, and then six groups were hatched 24 hours together. At last centrifuged, got together and then distilled the total RNA, It can be measured the ex- pression of bax, bcl -2 And caspase -3 by using the methods of RT -PCR and agarose gel electrophoresis. Results ：The group of decoration of Rehmarmiae cerebrospinal fluid can effectively reduse the expression of bax And caspase - 3 Gene and enhance the expression of bcl -2 ,so controlled phoned and restrained Apoptosis, enhanced living of Cells pc12.
出处
《中华中医药学刊》
CAS
2007年第9期1770-1773,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30472128)
