摘要
目的探讨用灯盏乙素定量人血浆中异灯盏乙素的LC/MS/MS方法。方法用AgiLent SB-C18柱(4.6 mm×150 mm,5μm),流动相为甲醇∶水∶甲酸(55∶43∶2)。采用电喷雾离子源(ESI),以选择反应监测(SRM)方式对灯盏乙素和异灯盏乙素及其苷元进行一级和二级质谱分析,离子反应分别为m/z 463→287和m/z 287→123。结果根据所得色谱和质谱数据,推断灯盏乙素可能的代谢产物和异灯盏乙素的结构,并测定人血浆中异灯盏乙素的浓度。结论用灯盏乙素定量异灯盏乙素的方法是可行的。
Objective To discuss the LC/MS/MS method for determination isoscutellarin concentration in human plasma by using scutellarin. Methods Using Agilent SB-C18 column（4.6 mm×150 mm,5 μm）,the mobile phase consisted of methanol-water-formic acid（55：43：2）.Using electrospray ionization source（ESI） and Selected reaction monitoring（SRM） mode,LC/MS/MS was applied to werify scutellarin,isoscutellarin and its aglycones, which were operated in negative ion mode and product ion mode.Ions fragment reaction were m/z 463à287 and m/z 287à123 respectively.Results The structure of scutellarin and its metabolized produces were deduced based on the chromatography and MS data,and isoscutellarin concentration in human plasma was determined.Conclusion The LC/MS/MS Method for Determination Isoscutellarin Concentration in Human Plasma by using Scutellarin can be applied.
出处
《空军总医院学报》
2007年第2期83-87,共5页
Journal of General Hospital of Air Force,PLA
