摘要
目的:观察丙戊酸钠对鼠坐骨神经切断后脊髓运动神经元凋亡及Bcl-2基因表达的影响。方法:采用清洁级SD大鼠30只,行右坐骨神经横形切断后即刻原位吻合术,制成周围神经损伤模型后,随机分为丙戊酸钠口服组(A组)、生理盐水对照组(B组)。分别于术后1,4,7,14,28 d取材,应用TUNEL法检测细胞凋亡数,用免疫组化技术检测脊髓运动神经元Bcl-2的表达,并对脊髓凋亡细胞以及阳性运动神经元进行计数。结果:坐骨神经切断后4,7,14 d,实验组脊髓运动神经元Bcl-2吸光度明显高于对照组(P<0.05);坐骨神经切断后7,14 d,实验组脊髓神经元凋亡率明显低于对照组(P<0.05)。结论:丙戊酸钠对鼠坐骨神经损伤后脊髓神经元具有保护作用。
Objective： To observe the effect of valproic acid（VPA） on bcl-2 expression and motor neuronal apoptosis in spinal cord of rats following sciatic nerve transection. Methods： Thirty SD rats were divided into VPA group and control group randomly according to the treatment after operation of sciatic nerve transaction. The specimens were taken at 7, 14 and 28 d after operation. Bcl-2 expression levels in spinal cord were assayed with immunohistochemistry SP method and numbers of motor neurons apoptosis with the method of TUNEL. The apoptosis neurons and positive motor neurons were counted. Results： Bcl-2 expression at 4, 7 and 14 d were obviously more intensive in VPA group than in control group （P〈0.05） ; while the apoptosis rate of spinal cord motor neurons at 7 and 14 d was obviously lower in VPA group than in control group （P〈0.05）. Conclusion： VPA can increase expression of bcl-2 in spinal cord and reduce neuronal apoptosis following sciatic nerve injury in rats, and has obvious protective effect on motor neuron in spinal cord of rats.
出处
《武汉大学学报（医学版）》
CAS
2007年第5期605-607,I0002,共4页
Medical Journal of Wuhan University
