摘要
从越南斗鸡暴发的病例中分离出1株新城疫病毒（NDV），命名为贵州分离株（DQ）。采用RT—PCR方法扩增分离株（DQ）的整个F基因片段，并将其克隆到pMD-18T载体中。序列分析结果表明，DQ株F基因分子长为1662bp，编码553个氨基酸。该毒株与国内2株分离株（CH2000、TW2000）之间的亲缘关系较近，其核苷酸和氨基酸序列同源性分别为95．7％-96．6％和96．6％-96．8％；但与LaSota、B1及1748E9等常见毒株的核苷酸和氨基酸同源性仅为84．0％-86．5％和87．2％-89．9％。DQ分离株在F蛋白裂解住点的氨基酸顺序为^112R—R—Q—K—R—F^117，与NDV强毒株特征相符，且具有101处的K（赖氨酸）和121处V（缬氨酸）2个特征性氨基酸，与NDV基因Ⅶ型的特性完全相符。利用MegAlign软件绘制NDV的系统发育进化树表明该分离株为基因Ⅶd亚型。
A strain of newcastle disease virus （NDV） was isolated from affected fighting-cock in Guizhou and designated by the name of DQ strain. The entire fragment of F gene of the isolate was amplified by RT - PCR and further cloned into pMD -18T vector. The sequence analysis showed that the nucleotide sequence of the F fragment was 1 662bp,coding for 553 amino acid. The isolate was closer to two Chinese strains（ CH2000 ,TW2000） in genetic relationship and nueleotide and amino acid sequence homologies were found to be 95.7% - 96.6% and 96.6% - 96.8% respectively. But the nucleotide and the amino acid sequence homologies to other NDV strains such as LaSota, B1 and F48E9 were only 84.0%-86.5% and 87.2%-89.9% respectively. The deduced amino acid sequence of the cleavage site of F protein Was^112R - R - Q - K - R - F^117 , conforming to the vindent NDV strains. The isolate possessed KWl and V121 , which was similar to the characteristic of genotype Ⅶ. The NDV systemic evolution tree was depicted by MegAlign software and showed that the DQ strain belonged to gene type Ⅶd.
出处
《山地农业生物学报》
2007年第4期300-304,共5页
Journal of Mountain Agriculture and Biology
基金
贵州省自然科学基金资助项目[黔科合字(2005)2030]
贵州大学人才科研基金资助项目[贵大人科合字(2006)018]
