摘要
目的探讨螺旋藻多糖(PSP)对MPTP制备的C57BL小鼠帕金森病(PD)模型多巴胺能神经元的保护作用。方法C57BL小鼠分5组:对照组、MPTP组、PSP高、中、低剂量预处理+MPTP组。用免疫组化、RT-PCR和HPLC-ECD方法检测黑质中酪氨酸羟化酶(TH)和多巴胺转运体(DAT)免疫反应阳性表达水平,TH和DAT mRNA表达水平及纹状体中多巴胺(DA)及其代谢产物3,4-二羟苯乙酸(DOPAC)和高香草酸(HVA)的含量。结果模型小鼠黑质中TH和DAT免疫阳性细胞数目减少,TH和DAT mRNA表达水平降低,纹状体中DA、DOPAC和HVA含量降低(P<0.01)。PSP预处理组与模型组相比,TH和DAT免疫阳性细胞数目增加,TH和DAT mRNA表达升高,DA、DOPAC和HVA含量升高(P<0.05)。结论PSP对MPTP所致C57BL小鼠PD模型的多巴胺能神经元有保护作用。
Aim The present study is designed to elucidate the protective effect of Polysaccharide from Spirulina Platensis（PSP） on dopaminergic neurons of MPTP-induced Parkinsons disease model in C57BL mice.Methods C57BL mice were divided into 5 groups：control group,MPTP group,PSP 200 mg·kg^-1 plus MPTP group,PSP 400 mg·kg^-1 plus MPTP group,and PSP 800 mg·kg^-1 plus MPTP group.Immunohistochemistry assay,RT-PCR,and HPLC-ECD were used to detect the immunoreactivity of tyrosine hydroxylase（TH） and dopamine transporters（DAT）,the expression of TH mRNA and DAT mRNA in substantia nigra（SN）,the content of dopamine（DA） and its metabolites,i.e.3,4-dihydroxyphenyl acetic acid（DOPAC） and homovanilic acid（HVA） in striatum（Str）.Results The result showed that,compared with control group,the TH and DAT immunoreactive stain in MPTP group was significantly decreased（P〈0.01）,the expression of TH mRNA and DAT mRNA were significantly decreased（P〈0.01） in SN,and the content of DA,DOPAC and HVA in Str was decreased（P〈0.01）.While in PSP 800 mg·kg^-1 pretreatment group,the immunoreactive stain of TH and DAT in SN were increased（P〈0.05）;the expression of TH mRNA and DAT mRNA were increased（P〈0.05）;the content of DA,DOPAC and HVA in Str was increased compared with MPTP group（P〈0.05）.Conclusion The results suggest that PSP plays a protective effect on MPTP-damaged dopaminergic neurons of C57BL mice.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2007年第9期1241-1245,共5页
Chinese Pharmacological Bulletin
基金
山东省科技厅资助课题(No031070125)
关键词
螺旋藻多糖
帕金森病
多巴胺能神经元
小鼠
polysaccharide from spirulina platensis
Parkinsons disease
dopaminergic neurons
mice
