摘要
目的观察芪丹通脉片联合骨髓间充质干细胞(MSCs)移植对急性心肌缺血大鼠心肌细胞凋亡、心功能的影响。方法采用密度梯度离心法体外分离培养大鼠MSCs,扩增至第3代后,应用溴脱氧尿嘧啶(BrdU)标记细胞。16只健康SD大鼠随机分为芪丹通脉片联合移植组与单纯移植组,分别灌服芪丹通脉片浸膏干粉溶液(2g/kg)和等体积生理盐水,每日1次,连续14d。结扎大鼠冠状动脉左前降支,制备急性心肌梗死模型,将BrdU标记的异体MSCs以微量注射的方法移植入缺血心肌内。移植后3h应用HP5500超声诊断仪,探头频率12MHz,检测大鼠心功能,4周后复查,并用免疫荧光法检测缺血区的微血管密度(MVD),用脱氧核苷酸转移酶(TdT酶)介导的dUTP缺口末端标记法(TUNNEL)检测心肌细胞凋亡指数(AI)。结果芪丹通脉片联合移植组与单纯移植组大鼠心功能均有所改善,而芪丹通脉片联合移植组改善更明显。其AI较单纯移植组明显减少,而缺血区MVD明显增加。结论芪丹通脉片联合MSCs移植能改善急性心肌缺血大鼠的心功能,减少心肌细胞凋亡,增加缺血区MVD,与MSCs单纯移植组比较疗效更显著。
Objective To observe the effect of marrow mesenchymal stem cells （MSCs） transplantation combined with Qidantongmai tablet and simple MSCs transplantation on myocardial cells apoptosis and heart function in acute myocardial ischemia rats. Method Rat MSCs were isolated by density gradient method, and then cultured and amplified to the third passage labeled with BrdU. 16 SD rats were divided into two groups randomly, transplantation combined with Qidantongmai tablet group and simple transplantation group. The isochoric of suspension of Qidantongmal tablet （2 g/kg） and NS were administered to SD rats by gastrogavage respectively for 14 days. The left anterior descending coronary artery of rat was ligated to establish animal models of acute myocardial infarction. The MSCs labeled with BrdU were implanted intramyocardially into the infarct area. After 3 hours, the heart function were detected by an echocardiographic system （HP 5500 system）, equipped with a 12 MHz probe. After 4 weeks, the heart function were rechecked and MVD were detected by immunofluorescence, myocardial cells apoptosis were detected by TUNNEL. Results Heart function were improved in both groups, and was more significant in MSCs transplantation combined with Qidantongmal tablet group. Apoptotic index were decreased and the MVD of ischemic zone were increased in transplantation combined with Qidantongmai tablet group than simple transplantation group.Conclusion MSCs transplantation combined with Qidantongmai tablet could improve heart function of acute myocardial ischemia rats, decrease apoptosis of myocardial cells, increase the MVD of ischemic zone, the therapeutic effect were more significant than simple MSCs transplantation.
出处
《中国中医药信息杂志》
CAS
CSCD
2007年第9期24-26,共3页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
芪丹通脉片
骨髓间充质干细胞
心功能
心肌细胞凋亡
大鼠
Qidantongmai tablet
marrow mesenchymal stem cells
heart function
apoptosis of myocardial cells
rat
