摘要
将人工合成的柞蚕抗菌肽D基因和天蚕抗菌肽B基因分别接上启动子和终止于，克隆到双元表达载体PBin19上，构建成双价抗菌肽基因表达载体pTBD。用三亲交配的方法将pTBD导入根癌农杆菌菌株LBA4404，再通过农杆菌将抗菌肽B、D基因导入广藿香，获27个转基因植株。Southern杂交结果表明，抗菌肽B、D基因已同时整合进3个株系染色体组中。与病原细菌Pseudomonassolanacearum试管共培养结果表明，转基因植株获得较强抗病性。盆栽接菌试验结果也表明．转基因植株具有较强抗病性。
The synthesized cecropin B and D genes were cloned into the binary vector pBinl9 after being linked to promoter and terminator separately, resulting in a bivalent cecropin gene ex-pression vector pTBDThe pTBDwas transferred into Agrobacterium tumefaciens strain LBA4404 via triparental mating. The transformed LBA4404 was then served to introduce the cecropin genes into patchouli (Pogostemon cablin, Benth. ) cells. Twentyseven transgenic plantlets werem obtained on the selective medium, three of which were confirmed to have both the cecropin B and D genes integrated into their chromosomes. The transgenic plantlets showed resistance to the pathogenic bacteria Pseudomonas solanacearum when co-cultured with the pathogenic .bacteria. The resistance of the transgenic plants was also observed in the soil watered with the path0genic - bacteria.
出处
《热带作物学报》
CSCD
1997年第1期50-57,共8页
Chinese Journal of Tropical Crops
关键词
广藿香
抗菌肽
双基因表达载体
转基因植株
Pogostemon cablin Benth
cecropin
double gene expression vector
transgenic plants
