摘要
目的:观察参芪健胃颗粒对急性炎症模型的抗炎作用。方法:实验于2006-02/05在河南中医学院动物实验中心完成。①将50只昆明小鼠随机均分为5组:大、中、小剂量的参芪健胃颗粒组(2.4,1.2,0.6g/kg),三九胃泰颗粒组(10g/kg)及生理盐水组。给药体积0.02mL/g。灌服给药1次/d,连续给药5d,于最后1次给药后1h,每鼠右耳涂二甲苯,4h后处死小鼠,沿耳廓基线解剖位置仔细剪下全耳,对齐双耳,修剪,使每耳大小一致,迅速用分析天平称重,并计算肿胀度(肿胀度=右耳重-左耳重)。②将40只SD大鼠随机均分为5组:大、中、小剂量的参芪健胃颗粒组(16,8,4g/kg),三九胃泰颗粒组(6.7g/kg)及同体积生理盐水组。给药体积2mL/kg。灌服给药1次/d,连续给药5d,于最后1次给药后立即在大鼠足趾肿胀仪上测大鼠正常左后足跖体积,给药后30min立即给每鼠左后足跖皮下注射0.1mL新配制的10%新鲜鸡蛋清液,分别于给蛋清后30,60,120,240,360min再次在大鼠足趾肿胀仪上测大鼠左后足跖体积,并计算肿胀率[足跖肿胀率=(给药后不同时间足跖体积-同侧正常足跖体积)/同侧正常足跖体积]。结果:50只小鼠和40只大鼠均进入结果分析。①大、中剂量的参芪健胃颗粒组和三九胃泰颗粒组耳壳肿胀度显著小于生理盐水组(P<0.01),小剂量参芪健胃颗粒组小于生理盐水组(P<0.05)。②与生理盐水组比,大剂量参芪健胃颗粒组和三九胃泰颗粒组在给药后30～60min均可明显抑制大鼠蛋清性足跖肿胀,使足跖肿胀率明显减小(P<0.05),在给药后120～360min均可显著抑制大鼠蛋清性足跖肿胀,使足跖肿胀度明显减小(P<0.01);中剂量参芪健胃颗粒组在给药后30～120min可明显抑制大鼠蛋清性足跖肿胀,使足跖肿胀率明显减少(P<0.05),在给药后240～360min可显著抑制大鼠蛋清性足跖肿胀,使足跖肿胀率显著减少(P<0.01);小剂量参芪健胃颗粒在给药后30～360min仅有抑制大鼠蛋清性足跖肿胀的趋势。结论:参芪健胃颗粒可显著抑制小鼠耳壳肿胀和大鼠足趾肿胀程度,有较好的抗炎作用。
AIM： To investigate the anti-inflammatory action of Shenqi Jianwei （SQJW） granules to the model of acute inflammation. METHODS： The experiment was performed in the animal experimental center of Henan University of Traditional Chinese Medicine from February to May 2006. （1）Fifty Kunming mice were randomly divided into high, middle, and low doses SQJW granules groups （2.4, 1.2, and 0.6 g/kg）, Sanjiu Weitai granules group （10 g/kg） and normal saline group. All groups were given 0.02 mL/g medicine, once daily for continuous 5 days. One hour after the last time administration, dimethylbenzene was smeared on the right ears of each mice, four hours later, all mice were executed, and their two ears were cut off carefully along the eadap baseline, then the two years were tnmmed and weighed quickly by the analytic balance to count the degree of tumefaction by the formula： tumefaction=weight of nght ear - weight of left ear. （2）Forty SD rats were randomly assigned to high, middle, and low doses SQJW granules groups （2.4, 1.2, and 0.6 g/kg）, Sanjiu Weitai granules group （6.7 g/kg） and normal saline group with eight animals in each group. All groups were given 2 mL/kg medicine, once daily for continuous 5 days. After the administration for the last time, the volume of normal left hind paw was measured; 30 minutes after the administration, 0.1 mL fresh liquid of egg white （10%） was subcutaneously injected to the left hind paw of all rats, and 30, 60, 120, 240, and 360 minutes later, the volume of paw were measured again to calculate the degree of tumefaction： [tumefaction degree= （volumes of paw at each time point-normal volume of paw at the same side）/the normal volume of paw at the same side]. RESULTS： Fifty mice and 40 rats were all involved in the result analysis. （1）The tumefaction degrees of ear in the high, and middle doses SQJW granules groups and the Sanjiu Weitai granules group were markedly decreased compared with that of the normal saline group （P 〈 0.01 ）, and the low dose SQJW granules group was slight decreased compared with the normal saline group （P 〈 0.05）. （2）Compared with the normal saline group, the high dose SQJW granules group and Sanjiu Weitai granules group inhibited the tumefaction degree of paw of the rats 30-60 minutes after the last time administration （P 〈 0.05）, and markedly inhibited the degree 120-360 minutes after the last time administration （P 〈 0.01）; The middle dose SQJW granules group lessened the tumefaction degree of paw 30-120 minutes after the last time administration （P 〈 0.05）, and markedly decreased the degree 240-360 minutes after the last time administration （P 〈 0.01）. The low dose SQJW granules only had the trend to restrain the tumefaction degree of the rat paw at 30-360 minutes after the last time administration. CONCLUSION： SQJW granules remarkably restrain the tumefaction degree of mice＇s eadap and rats＇ paws, and display good anti-inflammatory effect.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第38期7635-7638,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
教育部新世纪优秀人才支持计划(NCET-04-0657)
河南省高等学校创新人才培养工程(2004-23)~~
