摘要
为建立芦笋单倍体育种技术体系以解决国内芦笋新品种资源贫乏问题,该文以白芦笋花药为外植体进行了愈伤组织的诱导及绿芽分化研究。试验结果表明,芦笋花药经过4d的冷藏(4℃)处理后,接种于含有NAA2.0mg/L、6-BA 1.0mg/L及6%蔗糖的1/2MS培养基上,可产生愈伤组织;每年的4月份接种花药的愈伤组织诱导率最高,8月份次之,11月份最低。品种间、同一品种不同单株间花药的愈伤组织诱导率不同,以‘UC155’品种(18.9%)最高,显著高于其他三个品种‘UC142’(11.6%)、‘Gijnlim’(10.3%)、‘Thielim’(5.8%),所有试验株系中以‘UC155’-8的愈伤组织诱导率最高,达83.5%;每日补充光照强度为1000lx的光照,比全暗培养更有利于芦笋花药愈伤组织的形成;花药接种前进行离心处理(4000r/min)30min,不能提高愈伤组织诱导率;愈伤组织转瓶培养于NAA0.5mg/L、6-BA1.0mg/L及3%蔗糖的MS培养基上,能分化出无根绿芽;不同品种间绿芽分化率有差别,以‘Gijnlim’品种最高(67.2%),‘UC142’品种最低(14.1%);未分化绿芽的愈伤组织再转入于不含植物生长调节剂的MS培养基上培养,部分愈伤组织能分化出绿芽。
Experiments were conductedon the anther of Asparagus officinalis L. to study the callus inducement and green shoot differentiation. Results showed that callus of anther could be induced from the Asparagus anther which had been refrigerated at 4℃ for 4 days and then inoculated to 1/2 MS culture medium of NAsA 2.0 mg/L, 6-BA 1.0 mg/L and 6% sucrose. The optimal time to gather anther is April, in which the callus inducement rate arrived at the peak, while in August and November, the rates were lower. Among differ- ent breeds and plants, the callus inducement rate of anther varied, in which the breed of UC155 performed the highest inducement rate （18.9%）, distinctively higher than the other three breeds did, including UC142 （11.6%）, Gijnlim （10.3%） and ＇Thidim＇ （5.8%）; Of all the experiment strains, the breed of UC155-8 showed the highest inducement rate, which was 83.5%. Compared with dark culture, a supplement light of 1000 lx everyday could stimulate the callus inducement of White Asparagus anther. Centrifugal disposal （4000 r/min, 30 min） before inoculation of anther could not increase the inducement rate of callus. The callus which transferred to MS culture medium of NAA 0.5 mg/L, 6-BA 1.0 mg/L and 3% sucrose could differentiate green shoots without root. Differentiation rate varied among different breeds, in which the breed of ＇Gijnlim＇ differentiated most （67.2%） and breed ＇UC142＇ did least. Part of the non-differentiated callus could differentiate green shoots while transferred to MS culture medium without plant growth regulators.
出处
《中国农学通报》
CSCD
2007年第9期129-133,共5页
Chinese Agricultural Science Bulletin
基金
福建省自然科学基金项目"芦笋抗病超雄株培育技术的研究"(B0510035)
关键词
白芦笋
花药培养
愈伤组织
绿芽
Asparagus officinalis L., Anther culture, Callus, Green shoot
